Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4362378..4362875 Replicon chromosome
Accession NZ_CP092252
Organism Escherichia coli strain ISHS7

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MF614_RS21105 Protein ID WP_001173343.1
Coordinates 4362378..4362551 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MF614_RS21110 Protein ID WP_000398619.1
Coordinates 4362579..4362875 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MF614_RS21095 (4359048) 4359048..4360505 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
MF614_RS21100 (4360742) 4360742..4362259 + 1518 WP_001295074.1 lysine--tRNA ligase -
MF614_RS21105 (4362378) 4362378..4362551 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MF614_RS21110 (4362579) 4362579..4362875 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MF614_RS21115 (4363102) 4363102..4363374 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MF614_RS21120 (4363386) 4363386..4363616 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
MF614_RS21125 (4363797) 4363797..4365428 + 1632 WP_001216460.1 sensor histidine kinase -
MF614_RS21130 (4365425) 4365425..4366144 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T235816 WP_001173343.1 NZ_CP092252:c4362551-4362378 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T235816 NZ_LR134378:c1079882-1079460 [Lautropia mirabilis]
ATGATCCTCCTCGACACCAACGTCATCTCTGAACCCATGCGCCGGGAACCCGAGGCCCGGGTATTGGCCTGGCTGGACCG
GCAGCCGTCCGAAACCCTGTTCCTGTCCGCCATCACGGTTGCCGAGATCCGCAAGGGCATTGCATTGTTGCCAGCTGGCA
AGCGGCAGACCCTGCTGGCCGAGCGCCTGGACAAGCTGCTGCTGCCGATGTTCCATGGCCGCATCCTGCCTTTCGACATT
CACACGACACCGGCCTACGCCTCGGTCATTGCCCATGCAGCCGCAGCGGGTTGCACCATTGCCGCAGCTGACGGCTTCAT
CGCCGCCATCGCGCTGCAGCACCATTTCAGCGTGGCCACTCGCGACACCCACCCCTTTCAGGCGGCTGGGCTGGATGTCA
TCAATCCATGGACGACGGACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT235816 WP_000398619.1 NZ_CP092252:c4362875-4362579 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT235816 NZ_LR134378:c1080133-1079879 [Lautropia mirabilis]
ATGGCCATGCTCACCGTCCGCAATCTGCCTGATGAAGTCCATCGTGCGCTGAAAGCGCGTGCAGCCTCCCGTGGGCACAG
CACGGAAGCCGAGGTACGTGCCATCCTGGCTGATGCCGTCAAACCTGCCGAGCGAATCCGACTGGGATCGCTGCTGGCCG
GCATCGGCCAGGAGGTCGGGCTGACCGATGAAGAATTCGCCATCTTCGAGAACGTCCGGGACAGGACATCCGCACCAACG
GTGGATCTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References