Detailed information of TA system TA235813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2503592..2504230
• Replicon: chromosome
• Accession: NZ_CP092252
• Organism: Escherichia coli strain ISHS7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: MF614_RS12295
• Protein ID: WP_000813794.1
• Coordinates: 2504054..2504230 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MF614_RS12290
• Protein ID: WP_001270286.1
• Coordinates: 2503592..2504008 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MF614_RS12270 (2498744)	2498744..2499685	-	942	WP_001251315.1	ABC transporter permease	-
MF614_RS12275 (2499686)	2499686..2500699	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
MF614_RS12280 (2500717)	2500717..2501862	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
MF614_RS12285 (2502107)	2502107..2503513	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
MF614_RS12290 (2503592)	2503592..2504008	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MF614_RS12295 (2504054)	2504054..2504230	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MF614_RS12300 (2504452)	2504452..2504682	+	231	WP_000494244.1	YncJ family protein	-
MF614_RS12305 (2504774)	2504774..2506735	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MF614_RS12310 (2506808)	2506808..2507344	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
MF614_RS12315 (2507436)	2507436..2508611	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2508651..2509799	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T235813 WP_000813794.1 NZ_CP092252:c2504230-2504054 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T235813 NZ_LR134375:412438-412704 [Veillonella dispar]
ATGGCTTATAAACTGGAGTTTTCTAAACGGTTTGACAAGCAATTTTCTAAACTCGATAAATCAACGCAACGTTATCTCTT
TAATTGGCTTATCAAGAATGTAGATAATGTAGAAAATCCTAGATATTCAGGAAAATCATTGACAGGCAATAAGACGGGGC
TGTGGCGTTATCGCATTGGTAATTATAGAGTCATTGCTGATATTAATGATGATAGATGTATTATTTTAGCGTTAGAAGTT
GGCCATCGAAAAGATATTTACAAATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT235813 WP_001270286.1 NZ_CP092252:c2504008-2503592 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT235813 NZ_LR134375:412227-412448 [Veillonella dispar]
ATGGCAAATATTTCAGTCCGTTTAAATGAACAAGAAGAAGAGTTATTTAAAACATATGCTGAGTTTATGGATGAAACACT
TTCTACTCTCTTTAAGAAGGCTTTACTAGAAAAGATTGAAGATGAATTTGATCTTAAGGTAGGTCAAAAAGCATTAGCAG
AATATAAGCAAGATCCTGTTACTTATTCTGTAGCGGAAATGCGTGCAAAATATGGCTTATAA

Structures

Toxin

• PDB: 6HPB