Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1328604..1329229 Replicon chromosome
Accession NZ_CP092252
Organism Escherichia coli strain ISHS7

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag MF614_RS06475 Protein ID WP_000911329.1
Coordinates 1328831..1329229 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag MF614_RS06470 Protein ID WP_000450524.1
Coordinates 1328604..1328831 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MF614_RS06445 (1324406) 1324406..1324876 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
MF614_RS06450 (1324876) 1324876..1325448 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
MF614_RS06455 (1325594) 1325594..1326472 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
MF614_RS06460 (1326489) 1326489..1327523 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
MF614_RS06465 (1327736) 1327736..1328449 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
MF614_RS06470 (1328604) 1328604..1328831 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
MF614_RS06475 (1328831) 1328831..1329229 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
MF614_RS06480 (1329376) 1329376..1330239 + 864 WP_001267498.1 neutral zinc metallopeptidase -
MF614_RS06485 (1330254) 1330254..1332269 + 2016 WP_000829293.1 tRNA cytosine(34) acetyltransferase TmcA -
MF614_RS06490 (1332343) 1332343..1333041 + 699 WP_000679823.1 esterase -
MF614_RS06495 (1333151) 1333151..1333351 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T235801 WP_000911329.1 NZ_CP092252:1328831-1329229 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T235801 NZ_LR134363:1858539-1858901 [Actinomyces slackii]
ATGATCCTCATAGACACCAACGTGTGGATAGACCACCTGCACGTCAGCGACAACCGTCTCGTGGCCCTCCTCGAGAGAGA
TGAGGCTGCCACCCACGAGGCCGTCCTCACCGAACTGGCGCTTGGATCTATCAAGAACCGTGAGAAGGTCCTCAACTCCC
TGGGATCTCTACGACGGGTGCCCCAGATCTCCGATTGCGAGGTCCGCTGGTTCACCGAGCGTCGCCGCCTATGGGGAAGG
GGCCTGTCCGCCGTAGACGTGCACCTACTTGCATCCGTTGTCGCCGAGCGGGGTGCGCTGCTGTGGACTAGGGATAAGCG
CCTGGCAGCTGCCGCCGACGAACTCGGGGTTGGTTATCGATAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT235801 WP_000450524.1 NZ_CP092252:1328604-1328831 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT235801 NZ_LR134363:1858319-1858528 [Actinomyces slackii]
ATGCGCACCACGATCACACTCGACGACGACCTTGTGGAACGAGCAAGCGAGTTGACAGGCATTACCGAGCGGGCTGCGCT
TGTGAGGCAGGCGGTCAGCACTCTCATCAGAGTGGAGTCTTCGCGGCGTCTCATCGCACTCGGTGGCTCCGACCCTGCCG
CCGAAGCGGCCCCGCGCCATCGAGGCATGCGGGACCGGCAGGATTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References