Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 899153..899807 Replicon chromosome
Accession NZ_CP092252
Organism Escherichia coli strain ISHS7

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag MF614_RS04430 Protein ID WP_000244772.1
Coordinates 899400..899807 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MF614_RS04425 Protein ID WP_000354046.1
Coordinates 899153..899419 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MF614_RS04400 (894441) 894441..895184 + 744 WP_000951964.1 SDR family oxidoreductase -
MF614_RS04405 (895241) 895241..896674 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
MF614_RS04410 (896719) 896719..897030 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
MF614_RS04415 (897194) 897194..897853 + 660 WP_000250274.1 hemolysin III family protein -
MF614_RS04420 (897930) 897930..898910 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MF614_RS04425 (899153) 899153..899419 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MF614_RS04430 (899400) 899400..899807 + 408 WP_000244772.1 protein YgfX Toxin
MF614_RS04435 (899847) 899847..900368 - 522 WP_001055874.1 flavodoxin FldB -
MF614_RS04440 (900480) 900480..901376 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MF614_RS04445 (901401) 901401..902111 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MF614_RS04450 (902117) 902117..903850 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T235799 WP_000244772.1 NZ_CP092252:899400-899807 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T235799 NZ_LR134360:1004423-1004773 [Staphylococcus condimenti]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCGCCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGGCCTGTTGT
AATTATACAAAACGACACTGGAAATAAGTATAGTCCAACAGTCATTGTAGCTGCAATGACTGGACGGATTAATAAAGCAA
AAATTCCTACTCATGTTGAAATTGAAAAAAGTAAATATAAATTGGACAAAGATTCAGTTATTTTACTAGAACAGATAAGA
ACAGTCGATAAAAAGCGACTTAAAGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT235799 WP_000354046.1 NZ_CP092252:899153-899419 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT235799 NZ_LR134360:1004259-1004426 [Staphylococcus condimenti]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTATGCACAGATGGCTGACTTAAA
TCTCTCCCTCGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAATGAATCATATTTAGCTTTTGACAAGG
ATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References