Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 305111..305742 Replicon chromosome
Accession NZ_CP092252
Organism Escherichia coli strain ISHS7

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag MF614_RS01430 Protein ID WP_001259388.1
Coordinates 305111..305386 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag MF614_RS01435 Protein ID WP_000593555.1
Coordinates 305383..305742 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MF614_RS01415 (300115) 300115..301182 + 1068 WP_000361467.1 HlyD family secretion protein -
MF614_RS01420 (301179) 301179..303914 + 2736 WP_000149124.1 ribosome-associated ATPase/putative transporter RbbA -
MF614_RS01425 (303914) 303914..305038 + 1125 WP_001216257.1 ABC transporter permease -
MF614_RS01430 (305111) 305111..305386 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
MF614_RS01435 (305383) 305383..305742 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MF614_RS01440 (305862) 305862..306263 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
MF614_RS01445 (306269) 306269..307075 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
MF614_RS01450 (307072) 307072..307836 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
MF614_RS01455 (307836) 307836..308669 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
MF614_RS01460 (308666) 308666..309610 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 298127..306263 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T235797 WP_001259388.1 NZ_CP092252:305111-305386 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T235797 NZ_LR134358:622981-623400 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT235797 WP_000593555.1 NZ_CP092252:305383-305742 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT235797 NZ_LR134358:622745-622981 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References