Detailed information of TA system TA235796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 133800..134371
• Replicon: plasmid unnamed1
• Accession: NZ_CP092208
• Organism: Leptospira weilii strain FMAS_RT1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FH581_RS22575
• Protein ID: WP_016758353.1
• Coordinates: 134030..134371 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6QJ35
• Locus tag: FH581_RS22570
• Protein ID: WP_004503974.1
• Coordinates: 133800..134036 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH581_RS22540 (FH581_017710)	129713..130012	-	300	WP_051017925.1	DUF5615 family PIN-like protein	-
FH581_RS22545 (FH581_017715)	130009..130245	-	237	WP_076621926.1	DUF433 domain-containing protein	-
FH581_RS22550 (FH581_017720)	130386..130673	-	288	WP_004494992.1	helix-turn-helix transcriptional regulator	-
FH581_RS22555 (FH581_017725)	130688..131687	+	1000	Protein_143	IS630 family transposase	-
FH581_RS22560 (FH581_017730)	131779..132615	+	837	WP_016760011.1	DUF4062 domain-containing protein	-
FH581_RS22565 (FH581_017735)	132879..133460	+	582	WP_004503973.1	hypothetical protein	-
FH581_RS22570 (FH581_017740)	133800..134036	+	237	WP_004503974.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FH581_RS22575 (FH581_017745)	134030..134371	+	342	WP_016758353.1	endoribonuclease MazF	Toxin
FH581_RS22580 (FH581_017750)	134641..135348	+	708	WP_004503975.1	HNH endonuclease	-
FH581_RS22585 (FH581_017755)	135465..135947	+	483	WP_239244750.1	hypothetical protein	-
FH581_RS22590 (FH581_017760)	136451..136744	-	294	WP_016760043.1	helix-turn-helix transcriptional regulator	-
FH581_RS22595 (FH581_017765)	136747..137076	-	330	WP_170875531.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FH581_RS22600 (FH581_017770)	137208..138722	-	1515	WP_249739388.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..156461	156461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.17 Da
• Isoelectric Point: 10.1024

>T235796 WP_016758353.1 NZ_CP092208:134030-134371 [Leptospira weilii]
MVKSRKYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVTIKLKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLVF

Nucleotide

• Length: 342 bp

>T235796 NZ_LR134358:45416-45598 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8757.05 Da
• Isoelectric Point: 6.4652

>AT235796 WP_004503974.1 NZ_CP092208:133800-134036 [Leptospira weilii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEEKLSKITKQNLHSEISTGNSIGKEAW

Nucleotide

• Length: 237 bp

>AT235796 NZ_LR134358:45700-46101 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Structures

Antitoxin

• AlphaFold DB: M6QJ35