Detailed information of TA system TA235792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 21773..22344
• Replicon: plasmid pLiSL17
• Accession: NZ_CP092204
• Organism: Leptospira weilii strain FMAS_PD2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FH586_RS21155
• Protein ID: WP_016758353.1
• Coordinates: 22003..22344 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6QJ35
• Locus tag: FH586_RS21150
• Protein ID: WP_004503974.1
• Coordinates: 21773..22009 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH586_RS21115 (FH586_21090)	16899..17087	-	189	WP_239244735.1	DUF5615 family PIN-like protein	-
FH586_RS21125 (FH586_21100)	17982..18218	-	237	WP_076621926.1	DUF433 domain-containing protein	-
FH586_RS21130 (FH586_21105)	18359..18646	-	288	WP_004494992.1	helix-turn-helix transcriptional regulator	-
FH586_RS21135 (FH586_21110)	18661..19660	+	1000	Protein_26	IS630 family transposase	-
FH586_RS21140 (FH586_21115)	19785..20588	+	804	WP_235595190.1	DUF4062 domain-containing protein	-
FH586_RS21145 (FH586_21120)	20852..21433	+	582	WP_004503973.1	hypothetical protein	-
FH586_RS21150 (FH586_21125)	21773..22009	+	237	WP_004503974.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FH586_RS21155 (FH586_21130)	22003..22344	+	342	WP_016758353.1	endoribonuclease MazF	Toxin
FH586_RS21160 (FH586_21135)	22614..23321	+	708	WP_004503975.1	HNH endonuclease	-
FH586_RS21165 (FH586_21140)	23438..23920	+	483	WP_239244750.1	hypothetical protein	-
FH586_RS21170 (FH586_21145)	24424..24717	-	294	WP_016760043.1	helix-turn-helix transcriptional regulator	-
FH586_RS21175 (FH586_21150)	24720..25049	-	330	WP_170875531.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FH586_RS21180 (FH586_21155)	25553..25843	+	291	Protein_35	curli production assembly/transport component CsgG domain protein	-
FH586_RS21185 (FH586_21160)	25903..26004	+	102	Protein_36	DUF2905 domain-containing protein	-
FH586_RS21190 (FH586_21165)	26010..26752	+	743	WP_239248354.1	IS5 family transposase	-
FH586_RS21195 (FH586_21170)	26772..27311	+	540	WP_239248333.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..169653	169653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.17 Da
• Isoelectric Point: 10.1024

>T235792 WP_016758353.1 NZ_CP092204:22003-22344 [Leptospira weilii]
MVKSRKYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVTIKLKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLVF

Nucleotide

• Length: 342 bp

>T235792 NZ_LR134355:2550945-2551277 [Mycolicibacterium chitae]
GTGAACCGAGGGGAGATCTGGACGGTAGCGGGGGGCATCTACGCGGGGAAGTCACGTCCGGCGGTGATCGTTCAGGATGA
CCTCTTTGACGCCACGGGTTCAGTGACGGTTGCGCCCATGACCAGCACGTTGTTGGATGCGCCGTTGATGCGCATTCGGA
TAACCGGCGGACAAGGTCGGCTATCGGGACTGGACCACGACAGCGACGTGATGATCGATCAGCTCACCACCGTCAGAAGG
TCGAACGTTCACGTCCGAGTCGGCCGGCTCACTGCGGAACAAGTCGTCGAGGTGGAGCGGGCGATGATGGCATTCCTTGG
ACTGGCCCGGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8757.05 Da
• Isoelectric Point: 6.4652

>AT235792 WP_004503974.1 NZ_CP092204:21773-22009 [Leptospira weilii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEEKLSKITKQNLHSEISTGNSIGKEAW

Nucleotide

• Length: 237 bp

>AT235792 NZ_LR134355:2550742-2550948 [Mycolicibacterium chitae]
ATGGCGGTGAGGGACAGGGTAGGCGAGTACAGGCGGCGGATGCGCGAGCGCGGCCTGCGGCCGCTGCAAGTCTGGGTGCC
GGACGTGCGCACTGAGAGTTTCGCCGCCGAGGCGCATCGCCAGGCTTCGTTGGTCGCAAGAGCGGACGAGAGCAACGACG
ACCAGGACTTTATCGAGGCTGTCTCGACGCCCTGGGATGAGGAGTGA

Structures

Antitoxin

• AlphaFold DB: M6QJ35