Detailed information of TA system TA235788

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 53870..54441
• Replicon: plasmid pLiSL16
• Accession: NZ_CP092201
• Organism: Leptospira weilii strain FMAS_PD2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FH586_RS00415
• Protein ID: WP_016758353.1
• Coordinates: 54100..54441 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6QJ35
• Locus tag: FH586_RS00410
• Protein ID: WP_004503974.1
• Coordinates: 53870..54106 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH586_RS00375 (FH586_00375)	48996..49184	-	189	WP_239244735.1	DUF5615 family PIN-like protein	-
FH586_RS00385 (FH586_00385)	50079..50315	-	237	WP_076621926.1	DUF433 domain-containing protein	-
FH586_RS00390 (FH586_00390)	50456..50743	-	288	WP_004494992.1	helix-turn-helix transcriptional regulator	-
FH586_RS00395 (FH586_00395)	50758..51757	+	1000	Protein_78	IS630 family transposase	-
FH586_RS00400 (FH586_00400)	51849..52685	+	837	WP_016760011.1	DUF4062 domain-containing protein	-
FH586_RS00405 (FH586_00405)	52949..53530	+	582	WP_004503973.1	hypothetical protein	-
FH586_RS00410 (FH586_00410)	53870..54106	+	237	WP_004503974.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FH586_RS00415 (FH586_00415)	54100..54441	+	342	WP_016758353.1	endoribonuclease MazF	Toxin
FH586_RS00420 (FH586_00420)	54711..55418	+	708	WP_004503975.1	HNH endonuclease	-
FH586_RS00425 (FH586_00425)	55535..56017	+	483	WP_239244750.1	hypothetical protein	-
FH586_RS00430 (FH586_00430)	56521..56814	-	294	WP_016760043.1	helix-turn-helix transcriptional regulator	-
FH586_RS00435 (FH586_00435)	56817..57146	-	330	WP_170875531.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57876	57876

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.17 Da
• Isoelectric Point: 10.1024

>T235788 WP_016758353.1 NZ_CP092201:54100-54441 [Leptospira weilii]
MVKSRKYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVTIKLKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLVF

Nucleotide

• Length: 342 bp

>T235788 NZ_LR134355:813804-814238 [Mycolicibacterium chitae]
GTGGCGAAGCTATCTGTCTCTGTGGACGTGCCGCTCCCACCCGAGCAGGCCTGGGAGTGCGCGGCTGATCTGTCCCGTTA
CAAGGAGTGGCTGACCATCCATCGGGTGTGGCGCAGCGCGCTGCCGGAAACGCTGGAAAAGGGCACCACCCTGGACTCGA
TCGTCGAGGTCAAGGGGATGGCCAACCGGGTCAAGTGGACGATCGTGCATTACAAGCCCCCGCAGGCGATGACGCTCAAC
GGCGACGGCCGCGGCGGCGTCAAGATCAAGCTGATGGCCAAGATCACGCCCAAGGGCGACGGGTCGGTGGTCACCTTCGA
CACCCACCTCGGCGGGCCGGCGCTGTTCGGGCCCATCGGGATGGTGGTCGCGGGCGCGCTCAAGGGCGACATCCGCGAGT
CGCTGAACAACTTTGTGAAGGTCTTCACGGGCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8757.05 Da
• Isoelectric Point: 6.4652

>AT235788 WP_004503974.1 NZ_CP092201:53870-54106 [Leptospira weilii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEEKLSKITKQNLHSEISTGNSIGKEAW

Nucleotide

• Length: 237 bp

>AT235788 NZ_LR134355:813578-813790 [Mycolicibacterium chitae]
ATGTCGTTCCTCGACAAGGCCAAGAACCTGCTCGGAAAGAACGCCGACAAGGTCGGCGGCGCGATCGACAAGGCCGGCGA
TTTCGTCGACCAGAAGACGGACGGCAAATACGCCGGGCACGTCGACAAGGCCCAGGACGCCGCCAAGGACGCGGTCCAGA
AGATGGATCCCACCCAGGCGGATCCCAACCATAAGGACACTCCGCCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: M6QJ35