Detailed information of TA system TA235787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 25393..25964
• Replicon: plasmid pLiSL16
• Accession: NZ_CP092201
• Organism: Leptospira weilii strain FMAS_PD2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FH586_RS00200
• Protein ID: WP_016758353.1
• Coordinates: 25623..25964 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6QJ35
• Locus tag: FH586_RS00195
• Protein ID: WP_004503974.1
• Coordinates: 25393..25629 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH586_RS00160 (FH586_00160)	20519..20707	-	189	WP_239244735.1	DUF5615 family PIN-like protein	-
FH586_RS00170 (FH586_00170)	21602..21838	-	237	WP_076621926.1	DUF433 domain-containing protein	-
FH586_RS00175 (FH586_00175)	21979..22266	-	288	WP_004494992.1	helix-turn-helix transcriptional regulator	-
FH586_RS00180 (FH586_00180)	22281..23280	+	1000	Protein_35	IS630 family transposase	-
FH586_RS00185 (FH586_00185)	23372..24208	+	837	WP_016760011.1	DUF4062 domain-containing protein	-
FH586_RS00190 (FH586_00190)	24472..25053	+	582	WP_004503973.1	hypothetical protein	-
FH586_RS00195 (FH586_00195)	25393..25629	+	237	WP_004503974.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FH586_RS00200 (FH586_00200)	25623..25964	+	342	WP_016758353.1	endoribonuclease MazF	Toxin
FH586_RS00205 (FH586_00205)	26234..26941	+	708	WP_004503975.1	HNH endonuclease	-
FH586_RS00210 (FH586_00210)	27058..27540	+	483	WP_239244750.1	hypothetical protein	-
FH586_RS00215 (FH586_00215)	28044..28337	-	294	WP_016760043.1	helix-turn-helix transcriptional regulator	-
FH586_RS00220 (FH586_00220)	28340..28669	-	330	WP_170875531.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FH586_RS00225 (FH586_00225)	29173..29463	+	291	Protein_44	curli production assembly/transport component CsgG domain protein	-
FH586_RS00230 (FH586_00230)	29523..29624	+	102	Protein_45	DUF2905 domain-containing protein	-
FH586_RS00235 (FH586_00235)	29630..30354	+	725	Protein_46	IS5 family transposase	-
FH586_RS00240 (FH586_00240)	30415..30702	+	288	WP_026054191.1	type II toxin-antitoxin system HigB family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57876	57876

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.17 Da
• Isoelectric Point: 10.1024

>T235787 WP_016758353.1 NZ_CP092201:25623-25964 [Leptospira weilii]
MVKSRKYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVTIKLKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLVF

Nucleotide

• Length: 342 bp

>T235787 NZ_LR134355:58040-58339 [Mycolicibacterium chitae]
GTGATGAGGCCAGGCGACGTCGCGCCGCGTCTCGACACCGAGCACGAGCTGTACGTCGCGGTGCTATCGAACTCAATGCA
TCTGACGGCCAACACCGGCCGTGTGATCACGTGCCCGTTCATCCCCGGCGAGATTCCCGCCGACACGATGGCGATGGTCG
TGGCGACGGAGCGGCCGCGCGGTGTCGTAGTACCCGAACTGGTCCAATGGCTTCCCGTGGTGGCGCTCGACGCGCCGATC
GGCAACATCGGTGCCGACGCGGTGCATGAAACGAAGACCGTCCTGACGGCTCTGTTATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8757.05 Da
• Isoelectric Point: 6.4652

>AT235787 WP_004503974.1 NZ_CP092201:25393-25629 [Leptospira weilii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEEKLSKITKQNLHSEISTGNSIGKEAW

Nucleotide

• Length: 237 bp

>AT235787 NZ_LR134355:57816-58043 [Mycolicibacterium chitae]
ATGGCCAAGGACAAGATATCGGTGACGGTGGACGCAGCCGTGCTCGCCGCGGCGGACGCTCACGCTGCCGCAGCCGGGCT
GAACCGGTCGGAAATGATCGAGCAGGCTCTGCGCAACGAGAGCGTGCGACTCGCGCTGGAGAGCTATACGACGCAAACCG
TCCCGATGCTCGACATCGACTCCTACGCGGGGATGATTTACCAAGCGAATCGGTCCGCTGGTCTGTGA

Structures

Antitoxin

• AlphaFold DB: M6QJ35