Detailed information of TA system TA235759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1004385..1006114
• Replicon: chromosome
• Accession: NZ_CP092182
• Organism: Alcaligenes faecalis strain T17

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: MF263_RS04615
• Protein ID: WP_239055204.1
• Coordinates: 1005461..1006114 (-)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: MF263_RS04610
• Protein ID: WP_239055203.1
• Coordinates: 1004385..1005461 (-)
• Length: 359 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MF263_RS04595 (MF263_04595)	1001073..1002032	-	960	WP_022983507.1	phenylacetic acid degradation operon negative regulatory protein PaaX	-
MF263_RS04600 (MF263_04600)	1002192..1003661	-	1470	WP_026485269.1	phospholipase D-like domain-containing protein	-
MF263_RS04605 (MF263_04605)	1003666..1003902	-	237	WP_153229335.1	hypothetical protein	-
MF263_RS04610 (MF263_04610)	1004385..1005461	-	1077	WP_239055203.1	macro domain-containing protein	Antitoxin
MF263_RS04615 (MF263_04615)	1005461..1006114	-	654	WP_239055204.1	DUF4433 domain-containing protein	Toxin
MF263_RS04625 (MF263_04625)	1006699..1007325	+	627	WP_009457902.1	L-threonylcarbamoyladenylate synthase	-
MF263_RS04630 (MF263_04630)	1007485..1008135	+	651	WP_022983510.1	site-2 protease family protein	-
MF263_RS04635 (MF263_04635)	1008215..1009120	+	906	WP_009457899.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MF263_RS04640 (MF263_04640)	1009117..1010250	+	1134	WP_009457893.1	outer membrane protein assembly factor BamC	-
MF263_RS04645 (MF263_04645)	1010680..1011000	+	321	WP_083054060.1	sigma factor-like helix-turn-helix DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 25342.00 Da
• Isoelectric Point: 8.8503

>T235759 WP_239055204.1 NZ_CP092182:c1006114-1005461 [Alcaligenes faecalis]
MAYDYSKTLNPQKALIWRIVHRDNIPWILGNNLHCGNSSAQATSWVTIGNTELIGKRGEREVPIAPGGVLNDYIPFYFTP
FSPMMINIHSGRGGVQRRPNEEIVILVSDLHRISAQKIPFVYTDKHAYYGWANFYTDIQHLHNIDWKILQTRDFRRDPND
LEKFERYQAEALIHQMLPVSALLGMICYTDDTKTTIERWLQKQKINIPVHTRPGWYF

Nucleotide

• Length: 654 bp

>T235759 NZ_LR134348:c1026813-1026457 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 359 a.a.
• Molecular weight: 40380.60 Da
• Isoelectric Point: 7.7476

>AT235759 WP_239055203.1 NZ_CP092182:c1005461-1004385 [Alcaligenes faecalis]
MINYTKGNLLTADVEALVNTVNTVGVMGKGIALMFKERFPKNMAEYALACKENRVTTGKMFITSTGELSGVKWIVNFPTK
QHWRAKSKIEWIIDGLKDLRLFIEQNDIKSIAIPPLGAGNGGLDWNDVKPLIEKTLGDLQNTEIQVYEPTSKYQNIAKNT
GVKKLTPARAMIAELIRRYWILGMECSLLEIQKLSWFLQRVIEVNNLKNELNLRFQAKNYGPYASNLGHLLNALDGSYLS
SDKRIPDCDPLDVIAFNDPQKEYIQAYLASEGKIYLPAIEKASEIINGFESPFGMELLSTVDWLLSTGSSEPSLDSIKEG
IKNWPAGERWAQRKLVIFNDESLSLALKHIISINFRYN

Nucleotide

• Length: 1077 bp

>AT235759 NZ_LR134348:c1027093-1026800 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA