Detailed information of TA system TA235754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 69293..69864
• Replicon: plasmid pLiSL15
• Accession: NZ_CP092170
• Organism: Leptospira interrogans strain FMAS_RT2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FH584_RS20535
• Protein ID: WP_137108924.1
• Coordinates: 69293..69634 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: FH584_RS20540
• Protein ID: WP_000443825.1
• Coordinates: 69628..69864 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH584_RS20500 (FH584_20500)	65116..65727	-	612	WP_000851075.1	hypothetical protein	-
FH584_RS20505 (FH584_20505)	66823..67023	-	201	WP_001206464.1	hypothetical protein	-
FH584_RS20510 (FH584_20510)	67338..67484	+	147	WP_002080465.1	hypothetical protein	-
FH584_RS20515 (FH584_20515)	67447..68172	+	726	WP_239033804.1	hypothetical protein	-
FH584_RS20520 (FH584_20520)	68175..68516	+	342	WP_000727287.1	hypothetical protein	-
FH584_RS20525 (FH584_20525)	68577..68843	+	267	WP_000844450.1	hypothetical protein	-
FH584_RS20530 (FH584_20530)	68843..69226	+	384	WP_239033802.1	hypothetical protein	-
FH584_RS20535 (FH584_20535)	69293..69634	-	342	WP_137108924.1	endoribonuclease MazF	Toxin
FH584_RS20540 (FH584_20540)	69628..69864	-	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FH584_RS20545 (FH584_20545)	70213..71004	-	792	WP_061243661.1	hypothetical protein	-
FH584_RS20550 (FH584_20550)	71507..71675	+	169	Protein_68	AraC family transcriptional regulator	-
FH584_RS20555 (FH584_20555)	71885..73024	+	1140	WP_239033778.1	leucine-rich repeat domain-containing protein	-
FH584_RS20560 (FH584_20560)	73296..74234	+	939	WP_061234895.1	Rpn family recombination-promoting nuclease/putative transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94947	94947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12975.22 Da
• Isoelectric Point: 10.1024

>T235754 WP_137108924.1 NZ_CP092170:c69634-69293 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKDYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAKFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T235754 NZ_LR134342:c1321049-1320684 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT235754 WP_000443825.1 NZ_CP092170:c69864-69628 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT235754 NZ_LR134342:c1320691-1320368 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F6HC33