Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 6850..7421 Replicon plasmid pLiSL13
Accession NZ_CP092168
Organism Leptospira interrogans strain FMAS_RT2

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag FH584_RS19410 Protein ID WP_000240418.1
Coordinates 6850..7191 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag FH584_RS19415 Protein ID WP_000443825.1
Coordinates 7185..7421 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH584_RS19375 (FH584_19375) 2671..3282 - 612 WP_000851075.1 hypothetical protein -
FH584_RS19380 (FH584_19380) 4378..4578 - 201 WP_001206464.1 hypothetical protein -
FH584_RS19385 (FH584_19385) 4747..5040 + 294 WP_229266377.1 hypothetical protein -
FH584_RS19390 (FH584_19390) 5003..5728 + 726 WP_188093849.1 hypothetical protein -
FH584_RS19395 (FH584_19395) 5731..6072 + 342 WP_239033757.1 hypothetical protein -
FH584_RS19400 (FH584_19400) 6133..6399 + 267 WP_000844450.1 hypothetical protein -
FH584_RS19405 (FH584_19405) 6399..6782 + 384 WP_000617796.1 hypothetical protein -
FH584_RS19410 (FH584_19410) 6850..7191 - 342 WP_000240418.1 endoribonuclease MazF Toxin
FH584_RS19415 (FH584_19415) 7185..7421 - 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH584_RS19420 (FH584_19420) 7770..8561 - 792 WP_061243661.1 hypothetical protein -
FH584_RS19425 (FH584_19425) 8961..9437 - 477 WP_000691822.1 hypothetical protein -
FH584_RS19430 (FH584_19430) 10365..10985 + 621 WP_002105527.1 hypothetical protein -
FH584_RS19435 (FH584_19435) 11010..11909 + 900 WP_239033758.1 hypothetical protein -
FH584_RS19440 (FH584_19440) 11916..12296 - 381 WP_000410390.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..84167 84167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T235750 WP_000240418.1 NZ_CP092168:c7191-6850 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T235750 NZ_LR134341:c1399160-1398891 [Streptococcus pseudoporcinus]
ATGACCTATAAGCTAGTTATTAGTGATGATGTCAAAAAGCAATTAAAGAAAATGGATAAGTATGTCGCTTTAATGCTTGT
TAAGGATATGAAGAAGCAATTAGACAACCTTAACAATCCGAGACAGTTGGGTAAAGCTTTAACTGGTCAATACAAAGGAT
TATGGCGATATCGTATAGGTAACTATCGTGTTATTTGTGATATCATTGATGATGAATTAGTTACAATCGCTATCAGTATT
GGGCATAGAAAAGACATCTATAAAAAATAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT235750 WP_000443825.1 NZ_CP092168:c7421-7185 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT235750 NZ_LR134341:c1399377-1399150 [Streptococcus pseudoporcinus]
ATGACTACCATATCATTAAGATTAAATGAACAAGAAGAAAAACTTTTTAAAAGCTATTCTTTTCATACAGGAAAAACATT
ATCAGAGCTATTTAAAACATCTTTAAAAGAACAGATTGAAGATCAATTAGACTATGAAGAAGGTATAAAAGCTCTTGAAA
GCTTTAAAAAGAACCCAGTTACTCATAGCATTGATGATATTATCATGGAGCTAGAGAATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References