Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 729451..730027 Replicon chromosome
Accession NZ_CP092166
Organism Leptospira interrogans strain FMAS_RT2

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag FH584_RS02825 Protein ID WP_000617906.1
Coordinates 729686..730027 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag FH584_RS02820 Protein ID WP_000844758.1
Coordinates 729451..729699 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH584_RS02805 (FH584_02805) 725110..727092 + 1983 WP_137170961.1 HD family phosphohydrolase -
FH584_RS02810 (FH584_02810) 727726..728184 - 459 Protein_557 hypothetical protein -
FH584_RS02815 (FH584_02815) 728338..728634 + 297 WP_000477061.1 helix-turn-helix transcriptional regulator -
FH584_RS02820 (FH584_02820) 729451..729699 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
FH584_RS02825 (FH584_02825) 729686..730027 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FH584_RS02830 (FH584_02830) 731052..732044 - 993 WP_137108480.1 aldolase/citrate lyase family protein -
FH584_RS02835 (FH584_02835) 732184..733119 - 936 WP_000004530.1 ornithine carbamoyltransferase -
FH584_RS02840 (FH584_02840) 733903..734370 + 468 WP_000060053.1 hypothetical protein -
FH584_RS02845 (FH584_02845) 734367..734771 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T235749 WP_000617906.1 NZ_CP092166:729686-730027 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T235749 NZ_LR134340:c3518418-3518014 [Escherichia marmotae]
GTGGTCCTGTGGCAATCTGAATTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCCTGCTGATTCATGGGCTGGTTGCCGC
AGTTATTTTACTCATGCCATGGCCACTTAGTTACACCCCGCTATGGCTGGTGTTGCTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATTCCCGTCAGGGAGAGATTCGCCTGCTGATGGATGGGCGTTTACGTTGGCAAGGACAG
GAATGGAGCATCGTCAAAACGCCGTGGATGATTAAAAGCGGCATGATGTTACGTTTACGTTCTGATAGCGGCAAACGTCA
GCATTTGTGGCTGGCAGCAGACAGCATGGACGAAGCCGAATGGCGTGATTTACGGCGAATTTTGTTACAGCAAGAGATAC
GATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT235749 WP_000844758.1 NZ_CP092166:729451-729699 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT235749 NZ_LR134340:c3518665-3518399 [Escherichia marmotae]
ATGGATATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGCGGTATGCGTGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGCGACGATCCGGACC
TGTTCAACTGGCTGATGAATCATGGTAAACCTGCCGATGCAGAACTGGAAATGATGGTCAGACTCATTCAGACACGGAAC
CGGGATCGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References