Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 305434..306062 Replicon chromosome
Accession NZ_CP092166
Organism Leptospira interrogans strain FMAS_RT2

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag FH584_RS01255 Protein ID WP_000281042.1
Coordinates 305434..305832 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag FH584_RS01260 Protein ID WP_001192482.1
Coordinates 305832..306062 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH584_RS01230 (FH584_01230) 301199..301966 + 768 WP_137108560.1 DUF1554 domain-containing protein -
FH584_RS01235 (FH584_01235) 302485..303311 + 827 Protein_244 hypothetical protein -
FH584_RS01240 (FH584_01240) 303567..304644 - 1078 Protein_245 hypothetical protein -
FH584_RS01245 (FH584_01245) 304755..305015 - 261 WP_239033688.1 hypothetical protein -
FH584_RS01250 (FH584_01250) 305037..305336 - 300 Protein_247 hypothetical protein -
FH584_RS01255 (FH584_01255) 305434..305832 - 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
FH584_RS01260 (FH584_01260) 305832..306062 - 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH584_RS01265 (FH584_01265) 306660..307241 - 582 WP_137170912.1 hypothetical protein -
FH584_RS01270 (FH584_01270) 307481..308629 - 1149 WP_137170914.1 hypothetical protein -
FH584_RS01275 (FH584_01275) 308814..309929 - 1116 WP_000353261.1 protein kinase -
FH584_RS01280 (FH584_01280) 309984..310337 - 354 WP_137108558.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T235748 WP_000281042.1 NZ_CP092166:c305832-305434 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T235748 NZ_LR134340:2472199-2472306 [Escherichia marmotae]
ATGACGCTCGCGCAGTTTGCCATAACTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATCACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT235748 WP_001192482.1 NZ_CP092166:c306062-305832 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT235748 NZ_LR134340:c2472139-2472084 [Escherichia marmotae]
CAAGATTAGCCCCCGTTCTGTTGTCAGGTTTGACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References