Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 248889..249517 Replicon chromosome
Accession NZ_CP092161
Organism Leptospira interrogans strain FMAS_AP5

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag FH593_RS00930 Protein ID WP_000281042.1
Coordinates 249119..249517 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q72P17
Locus tag FH593_RS00925 Protein ID WP_001192484.1
Coordinates 248889..249119 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH593_RS00895 (FH593_00895) 244031..244240 - 210 WP_000041838.1 hypothetical protein -
FH593_RS00900 (FH593_00900) 244372..245001 + 630 WP_001081629.1 S24 family peptidase -
FH593_RS00905 (FH593_00905) 245176..246099 + 924 WP_000680712.1 M28 family peptidase -
FH593_RS00910 (FH593_00910) 246360..247047 + 688 Protein_178 SH3 domain-containing protein -
FH593_RS00915 (FH593_00915) 247277..247954 + 678 WP_000071998.1 hypothetical protein -
FH593_RS00920 (FH593_00920) 248177..248521 + 345 WP_000392070.1 hypothetical protein -
FH593_RS00925 (FH593_00925) 248889..249119 + 231 WP_001192484.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH593_RS00930 (FH593_00930) 249119..249517 + 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
FH593_RS00935 (FH593_00935) 249615..249914 + 300 Protein_183 hypothetical protein -
FH593_RS00940 (FH593_00940) 249936..250163 + 228 WP_000130601.1 hypothetical protein -
FH593_RS00945 (FH593_00945) 250306..251382 + 1077 WP_001170569.1 hypothetical protein -
FH593_RS00950 (FH593_00950) 251383..251841 + 459 WP_001239004.1 GNAT family N-acetyltransferase -
FH593_RS00955 (FH593_00955) 252057..252917 - 861 WP_000682235.1 hypothetical protein -
FH593_RS00960 (FH593_00960) 253458..254225 - 768 WP_000636952.1 DUF1554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T235746 WP_000281042.1 NZ_CP092161:249119-249517 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T235746 NZ_LR134340:c2257376-2257200 [Escherichia marmotae]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCAATTCTGAAAC
AACTCGATTTGAGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8698.99 Da        Isoelectric Point: 9.8940

>AT235746 WP_001192484.1 NZ_CP092161:248889-249119 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEGREQPKSDKREKL

Download         Length: 231 bp

>AT235746 NZ_LR134340:c2257154-2256738 [Escherichia marmotae]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCCGAAGCGTTGAC
GCAGGGAGAAACGGTCGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAACTCATCCCGTTACCTTCGCCATTGAATAACAACGATCACTTTATTGAAGTACCTTTAAGTGTTGCTTCTAAGGTA
TTGCTGCTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2D7U1

References