Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 686991..687567 Replicon chromosome
Accession NZ_CP092156
Organism Leptospira interrogans strain FMAS_AP6

Toxin (Protein)


Gene name chpK Uniprot ID -
Locus tag FH594_RS02740 Protein ID WP_000617908.1
Coordinates 687226..687567 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag FH594_RS02735 Protein ID WP_000844758.1
Coordinates 686991..687239 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH594_RS02720 (FH594_02720) 682644..684626 + 1983 WP_000802799.1 HD family phosphohydrolase -
FH594_RS02725 (FH594_02725) 685268..685726 - 459 WP_000453267.1 hypothetical protein -
FH594_RS02730 (FH594_02730) 685880..686176 + 297 WP_000477061.1 helix-turn-helix transcriptional regulator -
FH594_RS02735 (FH594_02735) 686991..687239 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
FH594_RS02740 (FH594_02740) 687226..687567 + 342 WP_000617908.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FH594_RS02745 (FH594_02745) 688594..689586 - 993 WP_000041093.1 aldolase/citrate lyase family protein -
FH594_RS02750 (FH594_02750) 689726..690661 - 936 WP_000004530.1 ornithine carbamoyltransferase -
FH594_RS02755 (FH594_02755) 691465..691932 + 468 WP_000060053.1 hypothetical protein -
FH594_RS02760 (FH594_02760) 691929..692333 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12371.30 Da        Isoelectric Point: 9.7995

>T235745 WP_000617908.1 NZ_CP092156:687226-687567 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGKVEIGLKLVTGLD

Download         Length: 342 bp

>T235745 NZ_LR134340:2174159-2175481 [Escherichia marmotae]
ATGCCAAAACTTGTCACGTGGATGAACAACCAGCGGGTAGGCGAGTTGACGAAGCTGACCAACGGTGCCCATACCTTTAA
GTATGCGCCGGAGTGGTTGACCAACCGTTATGCCAGGCCATTGTCGCTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCTGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGCGACCGCATCGTTAAACGTTATCAT
GCTAAATCCAGGCAACCGTTTGATTTATTGTCAGAAATAGGGCGAGATAGCGTTGGCGCCGTGACGTTATTACCAGAAGA
CGAAACCGTGACGCGCCCAATAATGGTATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGGGAGGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACTGCGCTG
CTCAGAATGGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCATATCATTAAATTGCCGATTGGTGAAAT
CAGACAGCCCAATGCGACGCTCGATCTCAGTCAGAGTGTTGATAATGAGTATTACTGTCTGCTGCTGGCAAAAGAATTCG
GGTTGAATGTTCCCGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGCTTTGACAGGCGCTGG
AATACTGAACGAACGGTTTTACTTCGCTTGCCGCAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCTGACGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTATTCCAGTGGTTGATTGGCTCGACAGACGGCCATGCGAAAAATTTCTCAGTGTTTATCCAG
GCCGGTGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCGGGAATACATAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAGAAAAACGGAAATCGATAAAATATATCCGCGACATT
TTTTGGCGACAGCAAAGGCACTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGAGACTTTGCCAGAATGATTCCC
GCAGCATTGGATAACGTGAAGGCTTCATTACCGGCAGATTTTCCGGAGAACGTGTTGACGGCAATTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATATGGAATTAAGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT235745 WP_000844758.1 NZ_CP092156:686991-687239 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT235745 NZ_LR134340:2173893-2174159 [Escherichia marmotae]
ATGATGAGCTTTCCGATGATCTATAGCCCAACGCAATTAGCAAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATAAAACAGGCGACTATTTCCAATTTCGAAAATAACCCTGACAACACCACAC
TAACGACATTTTTTAAGATATTACAGTCACTTAATCTCTCGATGACGCTATGCGACACGAAAAATGACTCGCCAGAATCA
ACCGTTCAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References