Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 267116..267744 Replicon chromosome
Accession NZ_CP092156
Organism Leptospira interrogans strain FMAS_AP6

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag FH594_RS01140 Protein ID WP_000281042.1
Coordinates 267116..267514 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q72P17
Locus tag FH594_RS01145 Protein ID WP_001192484.1
Coordinates 267514..267744 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH594_RS01110 (FH594_01110) 262408..263175 + 768 WP_000636952.1 DUF1554 domain-containing protein -
FH594_RS01115 (FH594_01115) 263716..264576 + 861 WP_000682235.1 hypothetical protein -
FH594_RS01120 (FH594_01120) 264792..265250 - 459 WP_001239004.1 GNAT family N-acetyltransferase -
FH594_RS01125 (FH594_01125) 265251..266327 - 1077 WP_001170569.1 hypothetical protein -
FH594_RS01130 (FH594_01130) 266470..266697 - 228 WP_000130601.1 hypothetical protein -
FH594_RS01135 (FH594_01135) 266719..267018 - 300 Protein_224 hypothetical protein -
FH594_RS01140 (FH594_01140) 267116..267514 - 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
FH594_RS01145 (FH594_01145) 267514..267744 - 231 WP_001192484.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH594_RS01150 (FH594_01150) 268112..268456 - 345 WP_000392070.1 hypothetical protein -
FH594_RS01155 (FH594_01155) 268679..269356 - 678 WP_000071998.1 hypothetical protein -
FH594_RS01160 (FH594_01160) 269586..270273 - 688 Protein_229 SH3 domain-containing protein -
FH594_RS01165 (FH594_01165) 270534..271457 - 924 WP_000680712.1 M28 family peptidase -
FH594_RS01170 (FH594_01170) 271632..272261 - 630 WP_001081629.1 S24 family peptidase -
FH594_RS01175 (FH594_01175) 272393..272602 + 210 WP_000041838.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T235744 WP_000281042.1 NZ_CP092156:c267514-267116 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T235744 NZ_LR134340:1926906-1927009 [Escherichia marmotae]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8698.99 Da        Isoelectric Point: 9.8940

>AT235744 WP_001192484.1 NZ_CP092156:c267744-267514 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEGREQPKSDKREKL

Download         Length: 231 bp

>AT235744 NZ_LR134340:c1927023-1926778 [Escherichia marmotae]
CTGGCTTTATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCG
CTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTCCGCGCG
TAAAGTGGTCAGAAAAAAAGCGCGTTTATCATCCTCTAAAATGTTAAAAACCGCCCGTTCTGGTGAAAGTACCCGGGCGG
TTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2D7U1

References