Detailed information of TA system TA235742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 34795..35360
• Replicon: plasmid pLiSL8
• Accession: NZ_CP092155
• Organism: Leptospira interrogans strain FMAS_AP7

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A0F6H5W7
• Locus tag: FH595_RS19505
• Protein ID: WP_000237067.1
• Coordinates: 34795..35118 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F6H5X2
• Locus tag: FH595_RS19510
• Protein ID: WP_000648189.1
• Coordinates: 35112..35360 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH595_RS19475 (FH595_19475)	30780..31404	-	625	Protein_23	integrase core domain-containing protein	-
FH595_RS19480 (FH595_19480)	31507..32703	+	1197	WP_000575407.1	kelch repeat-containing protein	-
FH595_RS19485 (FH595_19485)	33097..33288	-	192	Protein_25	AraC family transcriptional regulator	-
FH595_RS19490 (FH595_19490)	33581..34342	-	762	Protein_26	hypothetical protein	-
FH595_RS19495 (FH595_19495)	34258..34437	+	180	WP_000536132.1	hypothetical protein	-
FH595_RS19500 (FH595_19500)	34502..34687	-	186	Protein_28	toxin-antitoxin system, toxin component	-
FH595_RS19505 (FH595_19505)	34795..35118	-	324	WP_000237067.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FH595_RS19510 (FH595_19510)	35112..35360	-	249	WP_000648189.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FH595_RS19515 (FH595_19515)	36302..36547	-	246	WP_000695324.1	hypothetical protein	-
FH595_RS19520 (FH595_19520)	36667..37155	-	489	WP_002131542.1	DUF4942 domain-containing protein	-
FH595_RS19525 (FH595_19525)	37287..37790	-	504	WP_000614121.1	hypothetical protein	-
FH595_RS19530 (FH595_19530)	38037..38531	+	495	WP_017858174.1	hypothetical protein	-
FH595_RS19535 (FH595_19535)	38780..39058	-	279	WP_000542660.1	hypothetical protein	-
FH595_RS19540 (FH595_19540)	39304..39459	-	156	WP_002131775.1	hypothetical protein	-
FH595_RS19545 (FH595_19545)	39609..39806	-	198	WP_239043567.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64942	64942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-104)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12096.52 Da
• Isoelectric Point: 10.6275

>T235742 WP_000237067.1 NZ_CP092155:c35118-34795 [Leptospira interrogans]
MVISQYEVYLINLDPTVGHGIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Nucleotide

• Length: 324 bp

>T235742 NZ_LR134340:570134-570421 [Escherichia marmotae]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCGTAGGAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTACACGGCATCGTAGTTATTCTGGTTGCGCATATCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATTAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9507.04 Da
• Isoelectric Point: 5.1749

>AT235742 WP_000648189.1 NZ_CP092155:c35360-35112 [Leptospira interrogans]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Nucleotide

• Length: 249 bp

>AT235742 NZ_LR134340:570408-570710 [Escherichia marmotae]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCTCTGGCGAA
TAAATCCGACGAGGATATTGATTACAGTGATATCCCTCCTTCCAGCGATGAGCAATGGTCAAACGCCGAACGGGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGGCCAGGTAAAGGC
TATCAAACCCGCCTGAATGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A0F6H5W7

Antitoxin

• AlphaFold DB: A0A0F6H5X2