Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 2772222..2772798 Replicon chromosome
Accession NZ_CP092152
Organism Leptospira interrogans strain FMAS_AP7

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag FH595_RS11095 Protein ID WP_000617906.1
Coordinates 2772457..2772798 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag FH595_RS11090 Protein ID WP_000844758.1
Coordinates 2772222..2772470 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH595_RS11075 (FH595_11075) 2767874..2769856 + 1983 WP_137170961.1 HD family phosphohydrolase -
FH595_RS11080 (FH595_11080) 2770497..2770955 - 459 WP_000453265.1 hypothetical protein -
FH595_RS11085 (FH595_11085) 2771109..2771405 + 297 WP_000477061.1 helix-turn-helix transcriptional regulator -
FH595_RS11090 (FH595_11090) 2772222..2772470 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
FH595_RS11095 (FH595_11095) 2772457..2772798 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FH595_RS11100 (FH595_11100) 2773823..2774815 - 993 WP_137108480.1 aldolase/citrate lyase family protein -
FH595_RS11105 (FH595_11105) 2774955..2775890 - 936 WP_000004530.1 ornithine carbamoyltransferase -
FH595_RS11110 (FH595_11110) 2776674..2777141 + 468 WP_000060053.1 hypothetical protein -
FH595_RS11115 (FH595_11115) 2777138..2777542 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T235740 WP_000617906.1 NZ_CP092152:2772457-2772798 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T235740 NZ_LR134339:1238693-1238974 [Corynebacterium minutissimum]
ATGATTCAGTCGTTTGCTAACCGAGACACCGAGCTTGTCTGGTTGCGCCAAGCGTCACCCCGGATCGATTCAAGAATTCA
CAAGACTGCAAATAGAAAACTTCATCAGCTTGATGCAGCAGTTTCCCTGCAATCGCTGCGTGTTCCGCCGGGGAACCGAT
TGGAAGCTTTGAAAGGTGATCGAAAGGGGATGTACAGCATTCGAGTCAATCAACAATGGAGGATTACTTTTCGCTGGACC
GAAGCGGGCCCGGCGGATGTGGCAATAGAGGATTATCACTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT235740 WP_000844758.1 NZ_CP092152:2772222-2772470 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT235740 NZ_LR134339:1238983-1239282 [Corynebacterium minutissimum]
ATGAGTACCAAGTTGTATCCACCTATTCATCCTGGAGAAGTACTCAACGAAGACTTCATTAAGGCATTTGGGATTACCCA
GCATAAGTTAGCTATGGCCATTGGAGTCCCTCCGCGCAGGATTAACGAGATCGTCCACGGCAAGCGCGGAATTACAGCAG
ACACTGCGCTGCGTTTAGGAAGGTATTTCGGGGTAGAACCGCAGTTCTGGGTGAACTTGCAAGGACGATACGAGCTCGAA
ATAGCAGAGGATTCAATCGCTGAAGAAGTCAGCAGTATTAAGCCGCTCGAAGTGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References