Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1596824..1597338 Replicon chromosome
Accession NC_012121
Organism Staphylococcus carnosus subsp. carnosus TM300

Toxin (Protein)


Gene name mazF Uniprot ID A0A3G4R0E2
Locus tag SCA_RS07995 Protein ID WP_075140914.1
Coordinates 1596824..1597174 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID B9DMI8
Locus tag SCA_RS08000 Protein ID WP_015900820.1
Coordinates 1597171..1597338 (-) Length 56 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SCA_RS07975 1593851..1594621 - 771 WP_015900815.1 RNA polymerase sigma factor SigB -
SCA_RS07980 1594596..1595075 - 480 WP_015900816.1 anti-sigma B factor RsbW -
SCA_RS07985 1595077..1595403 - 327 WP_015900817.1 anti-sigma factor antagonist -
SCA_RS07990 1595479..1596480 - 1002 WP_015900818.1 PP2C family protein-serine/threonine phosphatase -
SCA_RS07995 1596824..1597174 - 351 WP_075140914.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
SCA_RS08000 1597171..1597338 - 168 WP_015900820.1 hypothetical protein Antitoxin
SCA_RS08005 1597419..1598573 - 1155 WP_015900821.1 alanine racemase -
SCA_RS08010 1598637..1598990 - 354 WP_015900822.1 holo-ACP synthase -
SCA_RS08015 1598992..1599492 - 501 WP_115314364.1 PH domain-containing protein -
SCA_RS08020 1599482..1600969 - 1488 WP_015900824.1 PH domain-containing protein -
SCA_RS08025 1600962..1601432 - 471 WP_015900825.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13075.18 Da        Isoelectric Point: 10.0708

>T23574 WP_075140914.1 NC_012121:c1597174-1596824 [Staphylococcus carnosus subsp. carnosus TM300]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIQISLGLTHRN

Download         Length: 351 bp

>T23574 NC_012121:c1597174-1596824 [Staphylococcus carnosus subsp. carnosus TM300]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGGCCTGTTGT
AATTATACAAAATGACACTGGAAATAAGTATAGTCCGACAGTTATTGTGGCAGCAATTACTGGACGGATTAATAAAGCAA
AAATTCCTACTCATGTTGAAATTGAAAAAAGTAAATATAAATTGGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTCGATAAAAAGCGACTTAAAGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin


Download         Length: 56 a.a.        Molecular weight: 6211.75 Da        Isoelectric Point: 3.6836

>AT23574 WP_015900820.1 NC_012121:c1597338-1597171 [Staphylococcus carnosus subsp. carnosus TM300]
MSTFNQNRSYSLEQSLKEGYAQMADLNLSLATEAFSVECEACDCNESYLAFDKDE

Download         Length: 168 bp

>AT23574 NC_012121:c1597338-1597171 [Staphylococcus carnosus subsp. carnosus TM300]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTATGCACAGATGGCTGACTTAAA
TCTCTCCCTCGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAATGAATCATATTTAGCTTTTGACAAGG
ATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G4R0E2

Antitoxin

Source ID Structure
AlphaFold DB A0A380F5Y4

References