Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 2351178..2351806 Replicon chromosome
Accession NZ_CP092152
Organism Leptospira interrogans strain FMAS_AP7

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag FH595_RS09515 Protein ID WP_000281042.1
Coordinates 2351178..2351576 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag FH595_RS09520 Protein ID WP_001192482.1
Coordinates 2351576..2351806 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH595_RS09490 (FH595_09490) 2346910..2347677 + 768 WP_000636954.1 DUF1554 domain-containing protein -
FH595_RS09495 (FH595_09495) 2348196..2349056 + 861 WP_149792691.1 hypothetical protein -
FH595_RS09500 (FH595_09500) 2349312..2350388 - 1077 WP_001170569.1 hypothetical protein -
FH595_RS09505 (FH595_09505) 2350499..2350759 - 261 WP_239033688.1 hypothetical protein -
FH595_RS09510 (FH595_09510) 2350781..2351080 - 300 Protein_1882 hypothetical protein -
FH595_RS09515 (FH595_09515) 2351178..2351576 - 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
FH595_RS09520 (FH595_09520) 2351576..2351806 - 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FH595_RS09525 (FH595_09525) 2352404..2352985 - 582 WP_137170912.1 hypothetical protein -
FH595_RS09530 (FH595_09530) 2353225..2354373 - 1149 WP_137170914.1 hypothetical protein -
FH595_RS09535 (FH595_09535) 2354558..2355673 - 1116 WP_000353261.1 protein kinase -
FH595_RS09540 (FH595_09540) 2355728..2356081 - 354 WP_149792689.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T235739 WP_000281042.1 NZ_CP092152:c2351576-2351178 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T235739 NZ_LR134338:c5718754-5718404 [Brevibacillus brevis]
GTGATTGTCAAACGTGGCGATGTGTTTTTCGCGGACCTGTCCCCGGTCGTTGGCTCTGAGCAAGGAGGCGTACGGCCTGT
TTTAGTCATTCAAAACGACATTGGAAATCGATTTAGTCCGACGGTCATCGTCGCAGCCATTACGGCGCAGATTCAAAAGG
CGAAGCTGCCTACGCATGTGGAAATTGACGCAAAAACTTATGGATTTGATCGTGACTCCGTTATTCTATTGGAACAAATA
CGAACCATTGACAAACAACGTCTCACTGATAAAATTACGCATCTCGATGACGAGATGATGGATCGGGTTAATGAATCCTT
GCAAATCAGTTTAGGACTCATTGATTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT235739 WP_001192482.1 NZ_CP092152:c2351806-2351576 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT235739 NZ_LR134338:c5719057-5718758 [Brevibacillus brevis]
ATGGTTGGAGGTGGATTTGTCTTGTCGAAGTCAAACACGAAACGCATCATGATCAGCTTGCCACAACACTTGCTTCAGGA
AGTGGATGGCGTTATTCAAAAGGAAAAATCCAATCGCAGCGAGTTTATTCGTCAAGCGATGCATTTGTATTTGAAAGAGC
GAAAAAAACGTACAATCCGTGAGACCATGCAAAAAGGGTACATGGAGATGGCGAAGATCAATCTGAATATGGCATCGGAG
GCTTTTGGCGCTGAAGAAGAGGCTGACCATACTCTTGTCCGCTTAGTTAGCGGGGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References