Detailed information of TA system TA235737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 39402..39973
• Replicon: plasmid pLiSL
• Accession: NZ_CP092150
• Organism: Leptospira interrogans strain FMAS_PN2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q8F5A3
• Locus tag: FH597_RS01660
• Protein ID: WP_000240418.1
• Coordinates: 39632..39973 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: FH597_RS01655
• Protein ID: WP_000443825.1
• Coordinates: 39402..39638 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH597_RS01625 (FH597_01630)	34638..34807	-	170	Protein_21	AraC family transcriptional regulator	-
FH597_RS01630 (FH597_01635)	35163..36461	-	1299	WP_000558495.1	group II intron reverse transcriptase/maturase	-
FH597_RS01635 (FH597_01640)	37594..37842	+	249	WP_000648189.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FH597_RS01640 (FH597_01645)	37836..38159	+	324	WP_000237065.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FH597_RS01645 (FH597_01650)	38383..38595	+	213	WP_000447737.1	hypothetical protein	-
FH597_RS01650 (FH597_01655)	38674..39066	+	393	WP_239055737.1	hypothetical protein	-
FH597_RS01655 (FH597_01660)	39402..39638	+	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FH597_RS01660 (FH597_01665)	39632..39973	+	342	WP_000240418.1	endoribonuclease MazF	Toxin
FH597_RS01665 (FH597_01670)	40257..41042	+	786	WP_239055738.1	hypothetical protein	-
FH597_RS01670 (FH597_01675)	41437..42570	-	1134	Protein_30	IS3 family transposase	-
FH597_RS01675 (FH597_01680)	42625..42770	-	146	Protein_31	AraC family transcriptional regulator	-
FH597_RS01680 (FH597_01685)	42834..43144	+	311	Protein_32	integrase core domain-containing protein	-
FH597_RS01685 (FH597_01690)	43289..43774	-	486	WP_000022551.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63559	63559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12918.12 Da
• Isoelectric Point: 9.9595

>T235737 WP_000240418.1 NZ_CP092150:39632-39973 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T235737 NZ_LR134337:254307-254651 [Enterococcus faecium]
ATGGTAGATGTTCCAAGACAAGGTGATATTCTTTTATTGAACACAGCTCCCAGATCTGGCCATGAACAGACTGGAAGAAG
ACCTTATATTGTTCTTAGTCACGATATTATTGCAGATACTAGTAATGTGGTTATTGTTGCACCGATAACTACAACTAGTA
GAAATTACCCACTATACATTGAAATAACAGAAAATCATAAAATGAAAACACGCGGAAAAGTTATGTTAGATCAAATCACA
ACAATTGATTATGAAGCCAGGAACTGCAAATTTCTAGAAAAAGCTTCTGAAAAATTAATAGAAGAATTATTAACAAAAAT
ACGAGCTGTATTTCAAAAAGTATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT235737 WP_000443825.1 NZ_CP092150:39402-39638 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT235737 NZ_LR134337:254050-254313 [Enterococcus faecium]
ATGGAAATGATACCAATCAAAAAATGGGGAAATAGCAACGGACTTAGATTGCCAAAAAATTTAATGGAATTTCTGGAATT
AAAGACTGATGATCGAGTATCAATCAAAAGCGAAGATGTTAATGGAAAAAGACGATTAATTATCGAACCAGCAATTTCAA
AAGAAGAAGTAACTATTGAAGAGCTTTTCAAAGATTACACAGAGGGCAAAGTAAATATTAAAATTCAGGATTTAGGAAGC
CCAGTAGGGAGAGAACTATGGTAG

Structures

Toxin

• AlphaFold DB: A0A4D8SL30

Antitoxin

• AlphaFold DB: A0A0F6HC33