Detailed information of TA system TA235730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2583957..2585545
• Replicon: chromosome
• Accession: NZ_CP092121
• Organism: Klebsiella quasipneumoniae subsp. similipneumoniae strain IF3SW-P1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: MEO32_RS12555
• Protein ID: WP_064187239.1
• Coordinates: 2583957..2585279 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: MEO32_RS12560
• Protein ID: WP_004205921.1
• Coordinates: 2585279..2585545 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEO32_RS12520 (2579039)	2579039..2579257	+	219	WP_002904808.1	ParD-like family protein	-
MEO32_RS12525 (2579238)	2579238..2580020	+	783	WP_065904745.1	type I methionyl aminopeptidase	-
MEO32_RS12530 (2580289)	2580289..2581404	+	1116	WP_065904746.1	serine hydrolase domain-containing protein	-
MEO32_RS12535 (2581391)	2581391..2581735	+	345	WP_004205915.1	hypothetical protein	-
MEO32_RS12540 (2581764)	2581764..2582297	+	534	WP_053810394.1	hypothetical protein	-
MEO32_RS12545 (2582300)	2582300..2582506	+	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
MEO32_RS12550 (2582648)	2582648..2583940	+	1293	WP_023318214.1	glycoside hydrolase family 10 protein	-
MEO32_RS12555 (2583957)	2583957..2585279	-	1323	WP_064187239.1	type II toxin-antitoxin system HipA family toxin	Toxin
MEO32_RS12560 (2585279)	2585279..2585545	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MEO32_RS12565 (2585828)	2585828..2586154	-	327	WP_053810395.1	hydroxyisourate hydrolase	-
MEO32_RS12570 (2586151)	2586151..2586651	-	501	WP_017900378.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
MEO32_RS12575 (2586629)	2586629..2588008	-	1380	WP_017900377.1	nucleobase:cation symporter-2 family protein	-
MEO32_RS12580 (2588354)	2588354..2589508	+	1155	WP_043519552.1	FAD-dependent urate hydroxylase HpxO	-
MEO32_RS12585 (2589489)	2589489..2590412	-	924	WP_004205929.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49026.36 Da
• Isoelectric Point: 8.6091

>T235730 WP_064187239.1 NZ_CP092121:c2585279-2583957 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARRTLPSDFSAHVWRAITENMLKLHARLQQGLLAG

Nucleotide

• Length: 1323 bp

>T235730 NZ_LR134335:3036014-3036268 [Yersinia enterocolitica subsp. palearctica]
GTGAGAATTATATTTTCCAGTTGTTCTTGGGAGGATTATCTTTATTGGCAACAAACGGATAAGAAAATCCTCAAACGCAT
TAATGAGTTAGTAAAAAATATTCAAAGAACGCCATTTGAGGGAAAGGGTAAACCGGAACCCCTTAAACATAATCTGGCAG
GGTTCTGGTCACGGAGGATTACAGAAGAGCACAGACTTGTTTATGAGGTTTCCGGTGATAATTTATTAATTGCTGCTTGT
CGTTACCATTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT235730 WP_004205921.1 NZ_CP092121:c2585545-2585279 [Klebsiella quasipneumoniae subsp. similipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT235730 NZ_LR134335:3035766-3036017 [Yersinia enterocolitica subsp. palearctica]
ATGAGAACAATTAGTTATAGTGAGGCGCGCCAGAATTTGTCGACAACGATGGTGCAAACGGTTGAGGATCGAGCCCCCAT
CCTCATCACTCGTCAAAATGGGACTTCTTGTGTTCTTATGTCACTTGAAGAATATGAATCATTGGAAGAAACTGCTTATT
TACTGCGCTCACCAGCAAACGCGAAGCATTTGATGGACTCAATTGAAGAGTTGAGAGCAGGAAAAGGAATTCAAAGGGAA
CTTGAAGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9