Detailed information of TA system TA235721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 104970..105541
• Replicon: plasmid p1_LIP1804061
• Accession: NZ_CP092114
• Organism: Leptospira noguchii strain IP1804061

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q8F5A3
• Locus tag: MAL06_RS20545
• Protein ID: WP_000240418.1
• Coordinates: 105200..105541 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: MAL06_RS20540
• Protein ID: WP_000443825.1
• Coordinates: 104970..105206 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL06_RS20510 (MAL06_20510)	100619..100963	+	345	WP_036026199.1	hypothetical protein	-
MAL06_RS20515 (MAL06_20515)	100950..101492	+	543	WP_061251579.1	hypothetical protein	-
MAL06_RS20520 (MAL06_20520)	101520..101669	+	150	WP_155717419.1	hypothetical protein	-
MAL06_RS20525 (MAL06_20525)	101662..101976	+	315	WP_002149298.1	hypothetical protein	-
MAL06_RS20530 (MAL06_20530)	102410..103699	+	1290	WP_061251578.1	hypothetical protein	-
MAL06_RS20535 (MAL06_20535)	103871..104668	+	798	WP_061251577.1	TIGR02391 family protein	-
MAL06_RS20540 (MAL06_20540)	104970..105206	+	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MAL06_RS20545 (MAL06_20545)	105200..105541	+	342	WP_000240418.1	endoribonuclease MazF	Toxin
MAL06_RS20550 (MAL06_20550)	105611..105980	-	370	Protein_92	hypothetical protein	-
MAL06_RS20555 (MAL06_20555)	105977..106255	-	279	WP_243820800.1	hypothetical protein	-
MAL06_RS20560 (MAL06_20560)	106924..107169	+	246	WP_004420603.1	ribbon-helix-helix domain-containing protein	-
MAL06_RS20565 (MAL06_20565)	107169..107573	+	405	WP_004420606.1	putative toxin-antitoxin system toxin component, PIN family	-
MAL06_RS21675	107613..107744	-	132	WP_279575927.1	hypothetical protein	-
MAL06_RS20570 (MAL06_20570)	107836..108956	-	1121	Protein_97	hypothetical protein	-
MAL06_RS20575 (MAL06_20575)	109097..109368	-	272	Protein_98	IS5/IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..127018	127018
	-	flank	IS/Tn	-	-	109120..109368	248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12918.12 Da
• Isoelectric Point: 9.9595

>T235721 WP_000240418.1 NZ_CP092114:105200-105541 [Leptospira noguchii]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T235721 NZ_LR134334:c6577871-6577578 [Pseudomonas chlororaphis]
ATGGAATACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCAGACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATAGGGGCGCCGGCTACCGCATCTATTTCACGGTTCGCGGCAACACCCTCATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT235721 WP_000443825.1 NZ_CP092114:104970-105206 [Leptospira noguchii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT235721 NZ_LR134334:c6577576-6577286 [Pseudomonas chlororaphis]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCCTTTGCCCCGGGCGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGTTGTGCACCACAGTGCCCCGAGGCTGA

Structures

Toxin

• AlphaFold DB: A0A4D8SL30

Antitoxin

• AlphaFold DB: A0A0F6HC33