Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 1572566..1573203 | Replicon | chromosome |
| Accession | NZ_CP092110 | ||
| Organism | Bacillus velezensis strain LOH112 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | LGL65_RS08115 | Protein ID | WP_003156187.1 |
| Coordinates | 1572566..1572916 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | LGL65_RS08120 | Protein ID | WP_003156188.1 |
| Coordinates | 1572922..1573203 (-) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LGL65_RS08075 (1567606) | 1567606..1568208 | - | 603 | WP_007410234.1 | PP2C family serine/threonine-protein phosphatase | - |
| LGL65_RS08080 (1568208) | 1568208..1568996 | - | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| LGL65_RS08085 (1568962) | 1568962..1569444 | - | 483 | WP_007609591.1 | anti-sigma B factor RsbW | - |
| LGL65_RS08090 (1569441) | 1569441..1569770 | - | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| LGL65_RS08095 (1569834) | 1569834..1570841 | - | 1008 | WP_007609589.1 | PP2C family protein-serine/threonine phosphatase | - |
| LGL65_RS08100 (1570853) | 1570853..1571254 | - | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| LGL65_RS08105 (1571257) | 1571257..1571622 | - | 366 | WP_007609584.1 | RsbT antagonist protein RsbS | - |
| LGL65_RS08110 (1571627) | 1571627..1572448 | - | 822 | WP_025649964.1 | STAS domain-containing protein | - |
| LGL65_RS08115 (1572566) | 1572566..1572916 | - | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| LGL65_RS08120 (1572922) | 1572922..1573203 | - | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| LGL65_RS08125 (1573323) | 1573323..1574492 | - | 1170 | WP_025649966.1 | alanine racemase | - |
| LGL65_RS08130 (1574609) | 1574609..1575616 | - | 1008 | WP_007410230.1 | outer membrane lipoprotein carrier protein LolA | - |
| LGL65_RS08135 (1575781) | 1575781..1576146 | - | 366 | WP_007609580.1 | holo-ACP synthase | - |
| LGL65_RS08140 (1576239) | 1576239..1576838 | + | 600 | WP_003156193.1 | rhomboid family intramembrane serine protease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T235717 WP_003156187.1 NZ_CP092110:c1572916-1572566 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T235717 NZ_LR134334:4719934-4720230 [Pseudomonas chlororaphis]
ATGCATATCATCACCGCCAAACGAATCTGGGAGGCCAAGAGTCGCTGGCCGCAATCGGCCAATGCGCTGGAGACCTGGTA
CAGAAAAGTCAAAAGCCTCAATCCGGCTGATTTTGCAGCTATCAAGGCGGTGTTTCCGGCCACCGACAAAGTGGGGGCGT
TTCACGTCTTCGATATTGGCGGCAACAAGTTACGAATCATCGCCGTGGTGCGTTATGAAGTGCGACGTCTCTATATCAGG
CACGTGCTGGACCATCACGAATACGACAAGGGCAGGTGGAAGGAGGAAACCAGATGA
ATGCATATCATCACCGCCAAACGAATCTGGGAGGCCAAGAGTCGCTGGCCGCAATCGGCCAATGCGCTGGAGACCTGGTA
CAGAAAAGTCAAAAGCCTCAATCCGGCTGATTTTGCAGCTATCAAGGCGGTGTTTCCGGCCACCGACAAAGTGGGGGCGT
TTCACGTCTTCGATATTGGCGGCAACAAGTTACGAATCATCGCCGTGGTGCGTTATGAAGTGCGACGTCTCTATATCAGG
CACGTGCTGGACCATCACGAATACGACAAGGGCAGGTGGAAGGAGGAAACCAGATGA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT235717 WP_003156188.1 NZ_CP092110:c1573203-1572922 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT235717 NZ_LR134334:4720227-4720628 [Pseudomonas chlororaphis]
ATGAATGTTCTGGTGAGGCAGGCGGCGGAGCATTGGCATTTCGTCGCCCCTGTGTTGCGTAAACCCAAGACCGAAGCCGA
TTACGATGCATTGGTCGTGGCGCTGGATGAACTGCTGGATCTCATGGGAGAGGACGAGAACCATCCAGTGACTTCGCTGG
TGGACATCATCGGCGATTGGATCGAAGCCTACGACGAAGAGCATCGACCGATGCCGATAGCCAGTGGCGTTGATGTCTTG
CGCTCCCTGATGCGCGAACATGGCCTGACCCAGAGCGATCTGCCAGGCGTGGGTGCGCAGTCGGTGGTTTCGGAAGTGCT
GAGCGGCAAGCGCCAGCTCAACCTGCGGCAGATCCGCTGGCTGGCCGAGCGCTTCGGTGTTTCGGCCGAGGTATTTATCT
AA
ATGAATGTTCTGGTGAGGCAGGCGGCGGAGCATTGGCATTTCGTCGCCCCTGTGTTGCGTAAACCCAAGACCGAAGCCGA
TTACGATGCATTGGTCGTGGCGCTGGATGAACTGCTGGATCTCATGGGAGAGGACGAGAACCATCCAGTGACTTCGCTGG
TGGACATCATCGGCGATTGGATCGAAGCCTACGACGAAGAGCATCGACCGATGCCGATAGCCAGTGGCGTTGATGTCTTG
CGCTCCCTGATGCGCGAACATGGCCTGACCCAGAGCGATCTGCCAGGCGTGGGTGCGCAGTCGGTGGTTTCGGAAGTGCT
GAGCGGCAAGCGCCAGCTCAACCTGCGGCAGATCCGCTGGCTGGCCGAGCGCTTCGGTGTTTCGGCCGAGGTATTTATCT
AA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|