Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/SpoIISA-SpoIISB |
| Location | 692514..693431 | Replicon | chromosome |
| Accession | NZ_CP092110 | ||
| Organism | Bacillus velezensis strain LOH112 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | I2HQ15 |
| Locus tag | LGL65_RS03300 | Protein ID | WP_007407256.1 |
| Coordinates | 692514..693260 (+) | Length | 249 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | I2HQ14 |
| Locus tag | LGL65_RS03305 | Protein ID | WP_003154807.1 |
| Coordinates | 693261..693431 (+) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LGL65_RS03280 (687917) | 687917..688852 | - | 936 | WP_237586758.1 | ring-cleaving dioxygenase | - |
| LGL65_RS03285 (689179) | 689179..690495 | + | 1317 | WP_007610842.1 | amino acid permease | - |
| LGL65_RS03290 (690782) | 690782..691399 | + | 618 | WP_003154804.1 | DUF47 domain-containing protein | - |
| LGL65_RS03295 (691412) | 691412..692410 | + | 999 | WP_017417610.1 | inorganic phosphate transporter | - |
| LGL65_RS03300 (692514) | 692514..693260 | + | 747 | WP_007407256.1 | type II toxin-antitoxin system SpoIISA family toxin | Toxin |
| LGL65_RS03305 (693261) | 693261..693431 | + | 171 | WP_003154807.1 | type II toxin-antitoxin system SpoIISB family antitoxin | Antitoxin |
| LGL65_RS03310 (693528) | 693528..693653 | + | 126 | WP_003154809.1 | hypothetical protein | - |
| LGL65_RS03315 (693688) | 693688..694566 | - | 879 | WP_007407257.1 | N-acetylmuramoyl-L-alanine amidase | - |
| LGL65_RS03320 (694580) | 694580..694843 | - | 264 | WP_003154813.1 | phage holin | - |
| LGL65_RS03325 (694857) | 694857..695120 | - | 264 | WP_021493821.1 | hemolysin XhlA family protein | - |
| LGL65_RS03330 (695172) | 695172..695933 | - | 762 | WP_025649639.1 | hypothetical protein | - |
| LGL65_RS03335 (695990) | 695990..696187 | - | 198 | WP_007610833.1 | XkdX family protein | - |
| LGL65_RS03340 (696192) | 696192..696563 | - | 372 | WP_017417608.1 | XkdW family protein | - |
| LGL65_RS03345 (696576) | 696576..696938 | - | 363 | WP_014721043.1 | hypothetical protein | - |
| LGL65_RS03350 (697049) | 697049..697600 | - | 552 | Protein_671 | terminase | - |
| LGL65_RS03355 (697713) | 697713..698225 | - | 513 | WP_017417605.1 | sigma-70 family RNA polymerase sigma factor | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 684574..710253 | 25679 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 249 a.a. Molecular weight: 29077.57 Da Isoelectric Point: 4.7755
>T235716 WP_007407256.1 NZ_CP092110:692514-693260 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG
Download Length: 747 bp
>T235716 NZ_LR134334:c4496163-4495735 [Pseudomonas chlororaphis]
ATGATAGTACTCGACACAAATGTGTTGTCAGAACTGATGCACCCTCAACCGGATACGGCGGTATTGCTGTGGATCGAAGC
GCAACCCGTCGAGGACCTCTACATCAGCGCCATGACCATGGCCGAGATTCTCCACGGTATCGCGCGTCTGCCCCACGGCA
AGCGCAAACAGACGCTGCATGCATCGGCGCTGGCCATGTTCGAGGAAGACTTCATCGACCGGATCGTGCCCTTCGGAACC
CAGGCGGCTCTTTACTACGCGCATCTGGTCTCGCATCGCGAAAGATCAGGCCAGCCGATCAACCTGGTGGACGCGCAGAT
TGCAGCGATATGCCGGGTCCATGACGCCGCGATTGCCACCCGCAACACCAAGGATTTCACCGACACTGGGCTGATCGTTA
TCAACCCCTGGCTACCGCTGGAAATCTGA
ATGATAGTACTCGACACAAATGTGTTGTCAGAACTGATGCACCCTCAACCGGATACGGCGGTATTGCTGTGGATCGAAGC
GCAACCCGTCGAGGACCTCTACATCAGCGCCATGACCATGGCCGAGATTCTCCACGGTATCGCGCGTCTGCCCCACGGCA
AGCGCAAACAGACGCTGCATGCATCGGCGCTGGCCATGTTCGAGGAAGACTTCATCGACCGGATCGTGCCCTTCGGAACC
CAGGCGGCTCTTTACTACGCGCATCTGGTCTCGCATCGCGAAAGATCAGGCCAGCCGATCAACCTGGTGGACGCGCAGAT
TGCAGCGATATGCCGGGTCCATGACGCCGCGATTGCCACCCGCAACACCAAGGATTTCACCGACACTGGGCTGATCGTTA
TCAACCCCTGGCTACCGCTGGAAATCTGA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6676.74 Da Isoelectric Point: 10.9273
>AT235716 WP_003154807.1 NZ_CP092110:693261-693431 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
Download Length: 171 bp
>AT235716 NZ_LR134334:c4496405-4496160 [Pseudomonas chlororaphis]
ATGGCCAGCCTTACGATCCGCAATCTGGACGACCACATCAAAGAACGCCTGCGCATCGAGGCCGCTCACAATGGTCATTC
CATGGAAGAAGAAGCCCGATTGATTTTGCGCCGCGCATTGAGCAGGACAGCTGACAGCCAAGGCCTGGGCAGCCGTATCA
GGGCACGCTTTGCCGAGACCGGCGGAGTAGAGTTGGAGCTGCCTGAACGTACCGAACCGGCACGCTTGGTGGACCTGGGC
GAATGA
ATGGCCAGCCTTACGATCCGCAATCTGGACGACCACATCAAAGAACGCCTGCGCATCGAGGCCGCTCACAATGGTCATTC
CATGGAAGAAGAAGCCCGATTGATTTTGCGCCGCGCATTGAGCAGGACAGCTGACAGCCAAGGCCTGGGCAGCCGTATCA
GGGCACGCTTTGCCGAGACCGGCGGAGTAGAGTTGGAGCTGCCTGAACGTACCGAACCGGCACGCTTGGTGGACCTGGGC
GAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|