Detailed information of TA system TA023571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 24917..25575
• Replicon: plasmid pHRM2a
• Accession: NC_012109
• Organism: Desulfobacterium autotrophicum HRM2

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: HRM2_RS24575
• Protein ID: WP_148214839.1
• Coordinates: 25243..25575 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: HRM2_RS24570
• Protein ID: WP_012663316.1
• Coordinates: 24917..25237 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRM2_RS24550	19952..20698	+	747	WP_012663313.1	PAS domain-containing protein	-
HRM2_RS25725	21262..23910	+	2649	WP_049770555.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
HRM2_RS24565	24333..24893	+	561	WP_012663315.1	recombinase family protein	-
HRM2_RS24570	24917..25237	-	321	WP_012663316.1	helix-turn-helix domain-containing protein	Antitoxin
HRM2_RS24575	25243..25575	-	333	WP_148214839.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRM2_RS26355	25991..26635	-	645	Protein_22	DUF4158 domain-containing protein	-
HRM2_RS24590	26783..27025	+	243	Protein_23	recombinase family protein	-
HRM2_RS25730	27201..27860	-	660	WP_012663322.1	response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	lnu(F)	-	1..68709	68709

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (8-101)

Antitoxin

• HigA2-like, helix-turn-helix domain (11-89)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12296.25 Da
• Isoelectric Point: 10.0046

>T23571 WP_148214839.1 NC_012109:c25575-25243 [Desulfobacterium autotrophicum HRM2]
MWVGSSKKDLMKMPDDVQDMFGFALHLAQSGQKHPDAKPLKGFGGAGVLEVVENYKTDTYRAVYTVRYTNAVYVVHCFQK
KSGQGIATPRPDMDLIKSRLKALDNFIKGK

Nucleotide

• Length: 333 bp

>T23571 NC_012109:c25575-25243 [Desulfobacterium autotrophicum HRM2]
ATGTGGGTAGGATCCAGCAAAAAGGATCTCATGAAAATGCCCGATGATGTGCAGGATATGTTTGGCTTTGCATTGCATCT
TGCTCAGTCGGGACAGAAACACCCCGATGCAAAACCACTTAAAGGATTTGGAGGAGCAGGAGTATTGGAAGTGGTGGAGA
ACTATAAAACAGACACATATCGGGCCGTTTATACCGTTCGATATACAAATGCAGTCTATGTGGTCCACTGTTTTCAAAAA
AAATCAGGCCAGGGTATAGCAACACCCAGGCCAGATATGGATTTGATCAAATCCCGCTTAAAGGCCTTAGATAATTTTAT
AAAAGGAAAATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11771.59 Da
• Isoelectric Point: 8.0067

>AT23571 WP_012663316.1 NC_012109:c25237-24917 [Desulfobacterium autotrophicum HRM2]
MIEIEKGSANVYEDLGRPNADRMFVKAILASKIGEIVRQRNLTQQQAAEILKIPQPKLSDLLRGQFRGISEAKMLTCLNY
LGSDVEIIIHTPGLDSSRGDTRVTFA

Nucleotide

• Length: 321 bp

>AT23571 NC_012109:c25237-24917 [Desulfobacterium autotrophicum HRM2]
ATGATTGAAATAGAGAAAGGCAGCGCCAATGTCTATGAGGATCTGGGAAGACCGAATGCCGACAGGATGTTTGTTAAGGC
AATACTGGCCTCAAAGATTGGAGAAATTGTTCGCCAGAGAAATTTAACTCAACAACAGGCAGCTGAAATATTAAAAATAC
CTCAACCCAAACTATCTGACCTTCTCCGGGGACAGTTCCGAGGGATCAGCGAGGCCAAGATGTTGACCTGTCTTAATTAT
TTGGGAAGTGATGTTGAAATTATTATACACACACCGGGATTAGATTCTTCAAGGGGAGATACCCGGGTAACCTTTGCCTG
A