Detailed information of TA system TA235698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 123533..124098
• Replicon: plasmid pLP337
• Accession: NZ_CP092103
• Organism: Rhodococcus qingshengii strain F2-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C3JNM9
• Locus tag: JKI97_RS31635
• Protein ID: WP_003943396.1
• Coordinates: 123742..124098 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JKI97_RS31630
• Protein ID: WP_151097304.1
• Coordinates: 123533..123745 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKI97_RS31615 (JKI97_31605)	119257..120051	+	795	WP_151097298.1	hypothetical protein	-
JKI97_RS31620 (JKI97_31610)	120367..121401	-	1035	WP_151097300.1	diiron oxygenase	-
JKI97_RS31625 (JKI97_31615)	121590..122837	-	1248	WP_030538129.1	IS256 family transposase	-
JKI97_RS32820	122862..122984	+	123	WP_257226873.1	hypothetical protein	-
JKI97_RS31630 (JKI97_31620)	123533..123745	+	213	WP_151097304.1	ribbon-helix-helix domain-containing protein	Antitoxin
JKI97_RS31635 (JKI97_31625)	123742..124098	+	357	WP_003943396.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JKI97_RS31640 (JKI97_31630)	124296..124685	-	390	WP_007728194.1	helix-turn-helix transcriptional regulator	-
JKI97_RS31645 (JKI97_31635)	125758..126210	-	453	WP_149357472.1	hypothetical protein	-
JKI97_RS31650 (JKI97_31640)	126496..126701	-	206	Protein_125	IS110 family transposase	-
JKI97_RS31660 (JKI97_31650)	128560..129000	-	441	WP_024488231.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..337428	337428
	-	inside	IScluster/Tn	-	-	121590..128100	6510

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-117)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-39)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12844.58 Da
• Isoelectric Point: 6.8817

>T235698 WP_003943396.1 NZ_CP092103:123742-124098 [Rhodococcus qingshengii]
MIRRAHIHWVDLDPAQGSEAAKRRPAVVVSNDRANATAENLGRGVITVVPLTTNTAHVFPFQVIVESDESGLARASKAQA
EQVRSVSVRRLDPEPVGKVGMETFAALEDALRLHLDLQ

Nucleotide

• Length: 357 bp

>T235698 NZ_LR134329:4444156-4444338 [Bordetella parapertussis]
ATGGACGGCAAAGAACTGATCAAGCAGCTTGAGCGGTCCGGGTGGACACTGCGGAGCGTTAAGGGCTCCCACCACGTTTT
CGCCCACCCAGACCGCCCCGGACATATCAGCGTCCCTCACCCTAAAAAGGATCTCGGCATCGGACTACTAAACAGCATCC
TCAAAGCAGCTGGTTTGAAGTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7668.40 Da
• Isoelectric Point: 4.1824

>AT235698 WP_151097304.1 NZ_CP092103:123533-123745 [Rhodococcus qingshengii]
MTTKFTISLPDEDAHLLDEYVRDHGLRGRSAGIRAALRLLSTSGLERDYAAAFAEGDVDEVWDAVSADTE

Nucleotide

• Length: 213 bp

>AT235698 NZ_LR134329:4444400-4444792 [Bordetella parapertussis]
GTGAAATATCCCATTGCCATTGAACCCGGCAGCGACACGCGCGCCTGGGGAGTCGTTGTTCCTGATCTGCCCGGCTGCTT
TTCCGCAGCGGACGAAGGGATTGATCAAGCAATCGAGAACGCCAAGGAAGCCATTGAGTTGTGGATTGAAACGGCCATCG
ACTCCGGCGCCCCCATTCCTGCGGCAACCAACATAGCCAATCATCAGGCCAATCCTGAGTTCGCCAACTGGATTTGGGCG
ATTGCTGATGTCGATCCTGCAGTCTTGGATGAAACTGTCGAGCGCGTGAACATCACAATCCCGCGCCGAATTCTCAAGCG
CCTGGATGATCGCGCCCGCGCCGCCGGCGAAAGCCGGAGCAGCTACATCGCACATCTCGCCATGACTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1F2PUJ5