Detailed information of TA system TA235696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 166610..167562
• Replicon: plasmid pCP209
• Accession: NZ_CP092100
• Organism: Rhodococcus qingshengii strain F2-2

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: JKI97_RS00885
• Protein ID: WP_241064675.1
• Coordinates: 166975..167562 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: JKI97_RS00880
• Protein ID: WP_241064674.1
• Coordinates: 166610..166978 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKI97_RS00860 (JKI97_00855)	161659..162729	-	1071	WP_241064671.1	PDDEXK nuclease domain-containing protein	-
JKI97_RS00865 (JKI97_00860)	163031..164987	+	1957	Protein_174	cutinase family protein	-
JKI97_RS00870 (JKI97_00865)	165234..165377	-	144	WP_241064672.1	hypothetical protein	-
JKI97_RS00875 (JKI97_00870)	165619..165840	+	222	WP_241064673.1	hypothetical protein	-
JKI97_RS00880 (JKI97_00875)	166610..166978	+	369	WP_241064674.1	ParD-like family protein	Antitoxin
JKI97_RS00885 (JKI97_00880)	166975..167562	+	588	WP_241064675.1	AAA family ATPase	Toxin
JKI97_RS00890 (JKI97_00885)	167804..170758	+	2955	WP_241064676.1	UPF0182 family protein	-
JKI97_RS00895 (JKI97_00890)	171017..171430	+	414	WP_241064677.1	hypothetical protein	-
JKI97_RS00900 (JKI97_00895)	171430..171998	+	569	Protein_181	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..209974	209974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (4-59)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21460.34 Da
• Isoelectric Point: 5.6227

>T235696 WP_241064675.1 NZ_CP092100:166975-167562 [Rhodococcus qingshengii]
VRRLDLVVGPNGAGKSTFVEQHLAPLFSGSVFVNADVIAKDRWPDAAEEHSYEAARIAATMRDALIERGEPFIAESHPSK
LELIDRARRAGFVVVLHVVLVPVELSVARVAERVAEGGHSVPEVKVRERFERLWPLVVDAIDRSDHATVCDNTAPDVTRI
VARFVDGDPVGETIWPAWTPSEFSRTPKWVSGPGQ

Nucleotide

• Length: 588 bp

>T235696 NZ_LR134329:c3288728-3288546 [Bordetella parapertussis]
ATGAACAGCACAGACCTGATCCAGCAACCGAAGGCCGACGGCTGGTATCGCGTGCATACCGTGGGTTCACACCACCAGTA
CAAGCACCCGACCAAGCCCGGCAAGGTCACCGTGCCGCATCCCAGGAAGGGCCTGCCGACAGCGACACAGCGCAGCATCC
TTAAGCAAGCCGGCCTGCGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13262.79 Da
• Isoelectric Point: 6.9922

>AT235696 WP_241064674.1 NZ_CP092100:166610-166978 [Rhodococcus qingshengii]
VVDKVTRISSELVDAAAAEGQREHRSARQQLEHWARVGREVSNQRQVARQRVEAARAGRIPLSVLSTEEGAVFNAEIAAT
LEESIAAGNHVADRAVQGRTTISLDEHGRVVKHLPDGSQIVL

Nucleotide

• Length: 369 bp

>AT235696 NZ_LR134329:c3288523-3288134 [Bordetella parapertussis]
ATGCTCTACCCGATCCATGTTTCCAAAGACGAAGGCAGCGCCTACGGGGCCGCCTTCCCTGATTTTCCCGGCTGCTTTGC
AGCCGCCGATGAACTCCAGGACCTCCCCCGCGCGGCGCAGGAAGCCGTTGAGGCGCACTTCTACGGAGAGACCGAGCGTA
TCCCCGCCCCGTCCGCCCCTGAAGCGTGGGCCAACGATGAGGACTATCAAGGCGGCTATTGGATGATGGTCGATATCGAC
CTGTCGAAGGTCAACACGAAGGCCGTGCGGCTCAACATCAGCCTGCCGGAAAACCTGGTTCACCGTATCGACGAGGAAGC
CAAGGCACGACGCCTTTCGCGCTCAGCGTTTCTGGCCATGGCTGCGGAGCACGAAATGGCCGACGCCTAG