Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 114537..115189 Replicon plasmid pP1-1
Accession NZ_CP092078
Organism Klebsiella pneumoniae strain P1-1

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag MDE60_RS25750 Protein ID WP_017901321.1
Coordinates 114537..114962 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag MDE60_RS25755 Protein ID WP_001261275.1
Coordinates 114959..115189 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MDE60_RS25715 (MDE60_25690) 110246..110362 + 117 Protein_115 transposase -
MDE60_RS25720 (MDE60_25695) 110487..110657 - 171 Protein_116 LysR family transcriptional regulator -
MDE60_RS25725 (MDE60_25700) 110668..110895 + 228 Protein_117 IS3 family transposase -
MDE60_RS25730 (MDE60_25705) 110987..112543 - 1557 WP_025999314.1 sensor domain-containing diguanylate cyclase -
MDE60_RS25735 (MDE60_25710) 112730..112903 - 174 Protein_119 nuclease -
MDE60_RS25740 (MDE60_25715) 112956..113492 - 537 Protein_120 integrase core domain-containing protein -
MDE60_RS25745 (MDE60_25720) 113551..114519 - 969 WP_102097429.1 IS5 family transposase -
MDE60_RS25750 (MDE60_25725) 114537..114962 - 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
MDE60_RS25755 (MDE60_25730) 114959..115189 - 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MDE60_RS25760 (MDE60_25735) 115442..118018 - 2577 WP_017901322.1 nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / tet(D) / blaCTX-M-14 / blaDHA-1 / qnrB4 - 1..241435 241435
- flank IS/Tn - - 113551..114519 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T235695 WP_017901321.1 NZ_CP092078:c114962-114537 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T235695 NZ_LR134329:c3154488-3154222 [Bordetella parapertussis]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGTGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATACCCGCCAGGTCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT235695 WP_001261275.1 NZ_CP092078:c115189-114959 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT235695 NZ_LR134329:c3154235-3153957 [Bordetella parapertussis]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References