Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 52315..53225 Replicon plasmid pP1-1
Accession NZ_CP092078
Organism Klebsiella pneumoniae strain P1-1

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag MDE60_RS25425 Protein ID WP_004144067.1
Coordinates 52755..53225 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag MDE60_RS25420 Protein ID WP_023287184.1
Coordinates 52315..52758 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MDE60_RS25400 (MDE60_25375) 47731..48480 + 750 Protein_52 IS5 family transposase -
MDE60_RS25405 (MDE60_25380) 48509..49477 + 969 WP_000654811.1 IS5 family transposase -
MDE60_RS25410 (MDE60_25385) 49673..51019 + 1347 WP_225517490.1 ThiF family adenylyltransferase -
MDE60_RS25415 (MDE60_25390) 51072..52214 + 1143 Protein_55 translesion error-prone DNA polymerase V subunit UmuC -
MDE60_RS25420 (MDE60_25395) 52315..52758 + 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
MDE60_RS25425 (MDE60_25400) 52755..53225 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
MDE60_RS25430 (MDE60_25405) 53312..54307 + 996 WP_077254794.1 IS5 family transposase -
MDE60_RS25435 (MDE60_25410) 54309..55277 + 969 WP_077253478.1 IS5-like element IS903B family transposase -
MDE60_RS25440 (MDE60_25415) 55466..55726 - 261 WP_017901409.1 hypothetical protein -
MDE60_RS25445 (MDE60_25420) 56031..56999 + 969 WP_250184869.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / tet(D) / blaCTX-M-14 / blaDHA-1 / qnrB4 - 1..241435 241435
- inside IScluster/Tn - - 45587..56999 11412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T235693 WP_004144067.1 NZ_CP092078:52755-53225 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T235693 NZ_LR134327:2047439-2048719 [Aggregatibacter aphrophilus ATCC 33389]
ATGAGTAACTCGATTCTAAACGTTGCCATGAATGGCTTGTTAGTCGGTGAATGGCGAAAACTCACCAGTGGCGCAACCGA
ATTTCAGTATGCAGAAAAATGGCTTGAATCTGTTCGCTCGCGTCCTATTTCATTGTCACTGCCCTTGTCGCACAAAATTT
ATCGAGGAGATTCCGTTTACAATTTCTTCGATAATCTCCTCCCGGACAATGAGCAGATTCGTTCACGCATTCAACAACGT
TTCCAAACCTCAACAAAATCCCCATTTGATTTACTTTCGGCGATTGGACAGGATTGTATCGGTGCAATTCAGCTTTATCA
GGGAGAAAGCCGATCAGTACGCCAAATTTATGCAGAACCGCTAAATGACAAAGAGGTGGAAAACGTACTGAAAAACTACC
GCACTTTTCCTCTCGGCATGGAAGAAAGCGAGGATTTCCGAATTTCTTTGGCCGGCGCTCAGGAAAAAACAGCGCTCCTT
TATTATCAGAATCAATGGCAGCGCCCTTTGCATTCCACCGCGACCAGCCATATTTTTAAACTTCCGATTGGTATGGTGGC
ACAAGGGCAACTTGATCTTTCTGGCAGCTGTGAAAATGAATGGCTTTGCTTGAATATTATGAAAGCCTTCGGTTTGCCGG
TTCCTCGAAGCCAAATTCAGTATTTCGGTGAAATAAAAGTGCTGATTGTGGAACGTTTTGATCGCCGTTGGTCTGAAAAC
AGAAGTTGGCTAATTCGTTTACCACAGGAAGATATGTGCCAAGCACTCAACATCGCCCCTGCGCGAAAATATGAAAATGA
CGGCGGGCCGAGCATTCTTCAAATTATGTCGCTTCTCAACGGTTCATCCAGCGCCCAAGCAGATCGCAAGCAGTTTTTTA
AAGCGCAAATTGTTTTTTGGCTACTTTGTGCCATTGACGGCCATGGCAAAAATTTCAGTTTATTTTTGGAACAAGAGAAT
CGCTATCGTCTAACGCCTTTTTATGACGTGATGTCCGCCTATCCACTTATCAAATATAACGGACTACAAAAGCAAAAAGT
CAAAATGGCCATGGCGTGGCACGGCGAAAATAAACATTATTTATGGGATAAAATACAGCTCCGCCACATTTTCAATACCG
CCAAACTCGCAGGCTTGGCAAAGGAAACCGTAGAAGATATTTTGCATGAAATCAGTGCACTATATCCAAGAATCAGTGAA
ATAAATACCCATGGATTGCCTGATGAAATTGTCGTTCCTATTTTCATGGGATTGGAAATGCAGATGAAAAAGATAAGTTA
A

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT235693 WP_023287184.1 NZ_CP092078:52315-52758 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT235693 NZ_LR134327:2047161-2047439 [Aggregatibacter aphrophilus ATCC 33389]
ATGGTTATCACCACCGCAAAAATGCTTTCTCATGTAATTCGTGAATTTCGCTATCAAGGCGATCTGTCACAAACTGATGT
TGCTAAACTCGCCGGAACGACCCAAGCCAGAATTTCAGCGTTTGAGAATGAGCCGGAGCGTACTAAGTTAGAAACGCTAT
TTAAAATTCTGGCAGGATTAGAACTAGAGCTTATTGTACAACCTCGCAAAAAATCATCTGATACTAATCTATCAATCAAA
GAGCCAACAGCAACATACGATGACGATGAGGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References