Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5112186..5112811 Replicon chromosome
Accession NZ_CP092077
Organism Klebsiella pneumoniae strain P1-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MDE60_RS24765 Protein ID WP_002882817.1
Coordinates 5112186..5112569 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MDE60_RS24770 Protein ID WP_004150355.1
Coordinates 5112569..5112811 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MDE60_RS24750 (MDE60_24725) 5109552..5110454 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MDE60_RS24755 (MDE60_24730) 5110451..5111086 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MDE60_RS24760 (MDE60_24735) 5111083..5112012 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MDE60_RS24765 (MDE60_24740) 5112186..5112569 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MDE60_RS24770 (MDE60_24745) 5112569..5112811 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MDE60_RS24775 (MDE60_24750) 5113016..5113933 + 918 WP_021466676.1 alpha/beta hydrolase -
MDE60_RS24780 (MDE60_24755) 5113947..5114888 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MDE60_RS24785 (MDE60_24760) 5114933..5115370 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MDE60_RS24790 (MDE60_24765) 5115367..5116227 - 861 WP_004146232.1 virulence factor BrkB family protein -
MDE60_RS24795 (MDE60_24770) 5116221..5116820 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T235692 WP_002882817.1 NZ_CP092077:c5112569-5112186 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T235692 NZ_LR134327:c365199-364861 [Aggregatibacter aphrophilus ATCC 33389]
GTGAAACTATCGTTTATTGAATTGCCGCCCTTTGAGCGTTACAGAAAAGCGTATTTGTCCGATGATGAATATAGGGCTTT
CCAGAATGAGCTATTAGAAAACCCCGAAAAGGGAGACGTGATACAGAACACGGGCGGATTGAGAAAAATTAGAATCGCTG
ACAGTGAGAGAAACAAAGGTAAACGTGGTGGCGCACGAGTGATTTATTACTATCTCATACGTAAATCACGGATATTACTC
GTTACTGCTTACAGTAAAAACAGATGCGAAGATTTAACCACAGAGCAATATAAAATTTTAGCTAAACTGGTGAAAGAGAT
AGAGGAGCTAGAGCAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT235692 WP_004150355.1 NZ_CP092077:c5112811-5112569 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT235692 NZ_LR134327:c364864-364556 [Aggregatibacter aphrophilus ATCC 33389]
ATGAGTGAATTATTTGATGAGCTTATAGAGGGGCTAAATGCCTATAAAGACTTTACACAGGGGAAAATAACGCTAAGAGT
AGAGACGCGTGAACGAATTGAACCTGCAAGCATTACGGCAGAGGAAGTGAAAGCAATTAGAGAGAAATTAAACCTTTCTC
AAGCTGTCTTTGCGCAGAAGTTACGAACCAGTGTAAGAACCTATCAAGGCTGGGAGCAAGGCAAAACCAAACCTAGCGCG
CACGCCTCTTTATTGTTGCGCATGGTGGATAAAGCCCCGCAAACCTTTGAGCTTATCGCAGGGATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References