Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4533822..4534632 Replicon chromosome
Accession NZ_CP092077
Organism Klebsiella pneumoniae strain P1-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag MDE60_RS22015 Protein ID WP_002887280.1
Coordinates 4533822..4534355 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MDE60_RS22020 Protein ID WP_002887278.1
Coordinates 4534366..4534632 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MDE60_RS22010 (MDE60_21985) 4532654..4533775 + 1122 WP_009309849.1 cupin domain-containing protein -
MDE60_RS22015 (MDE60_21990) 4533822..4534355 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
MDE60_RS22020 (MDE60_21995) 4534366..4534632 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MDE60_RS22025 (MDE60_22000) 4534735..4536168 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
MDE60_RS22030 (MDE60_22005) 4536158..4536841 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MDE60_RS22035 (MDE60_22010) 4537013..4538395 + 1383 WP_064153949.1 efflux transporter outer membrane subunit -
MDE60_RS22040 (MDE60_22015) 4538413..4538757 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T235689 WP_002887280.1 NZ_CP092077:c4534355-4533822 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T235689 NZ_LR134326:c2248725-2248300 [Bordetella bronchiseptica]
ATGATCCTGCTCGACACCGACGTGCTGCTCGAGCCGCTGCGGCCGGCCCCCGACCCGGCCGTCGCGGCATGGCTGGATGC
CCAGCACGTCGAAACCCTGTACCTGGCCGCGCCCGGCGCGGCCGAAATCCAGCTCGGGCTGGCCCGCCTGCCGCGCGGCA
AGCGGGCCGAGGCCCTGCGCCACGAATTCGAGCGGCGCGTCCTGCCGCTGTTCATGGGCCATGTCCTGCCCTTCGACACG
GCCGCGGCCGATGCCTACGCCGCCGTCATGATGCGGGCCGCGGCGGCGGGCCATGCGCTGTGCGCAATCGATGGCTGCAT
CGCCGCCATCGCCACCGCGCACGGCCTGGCCATCGCCACGCGCCATCCGGCCAGGTTCGAGGCGGCCGGGCTCGCCGCCG
TCGATCCCTGGCACGCCGCCGCTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT235689 WP_002887278.1 NZ_CP092077:c4534632-4534366 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT235689 NZ_LR134326:c2248961-2248722 [Bordetella bronchiseptica]
GTGTCTTCGATTACGGTAAGAAATGTTCCCGAGGAAGTGCATCGCGCCTTGCGCGTACGCGCGGCCACGCATGGGCGCAG
CACCGAGGCCGAGATCCGCGCCATCCTGGAAAACGCCGTGCTGCCCGATGGCCGCGTCAAGCTGGGCACCCTGCTCAGCC
ACATCGGCCGCGGCGCGCGCGGCATGGACCTGACCATCGAGCGCAATCCGGCGCCCGCCCGCCCGGCCAGTTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References