Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2607471..2609059 Replicon chromosome
Accession NZ_CP092077
Organism Klebsiella pneumoniae strain P1-1

Toxin (Protein)


Gene name hipA Uniprot ID W8UI36
Locus tag MDE60_RS12680 Protein ID WP_002904792.1
Coordinates 2607471..2608793 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag MDE60_RS12685 Protein ID WP_002904790.1
Coordinates 2608793..2609059 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MDE60_RS12665 (MDE60_12655) 2602527..2605028 + 2502 WP_102011944.1 fimbria/pilus outer membrane usher protein -
MDE60_RS12670 (MDE60_12660) 2605016..2606020 + 1005 WP_004190163.1 fimbrial protein -
MDE60_RS12675 (MDE60_12665) 2606162..2607454 + 1293 WP_064145447.1 glycoside hydrolase family 10 protein -
MDE60_RS12680 (MDE60_12670) 2607471..2608793 - 1323 WP_002904792.1 type II toxin-antitoxin system HipA family toxin Toxin
MDE60_RS12685 (MDE60_12675) 2608793..2609059 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
MDE60_RS12690 (MDE60_12680) 2609324..2609650 - 327 WP_002904788.1 hydroxyisourate hydrolase -
MDE60_RS12695 (MDE60_12685) 2609647..2610147 - 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
MDE60_RS12700 (MDE60_12690) 2610125..2611504 - 1380 WP_004184021.1 nucleobase:cation symporter-2 family protein -
MDE60_RS12705 (MDE60_12695) 2611850..2613004 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
MDE60_RS12710 (MDE60_12700) 2612985..2613908 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49171.46 Da        Isoelectric Point: 7.5357

>T235686 WP_002904792.1 NZ_CP092077:c2608793-2607471 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T235686 NZ_LR134326:c1946600-1946334 [Bordetella bronchiseptica]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGCGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATACCCGCCAGGTCTACGCCGAGACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTACGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT235686 WP_002904790.1 NZ_CP092077:c2609059-2608793 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT235686 NZ_LR134326:c1946347-1946069 [Bordetella bronchiseptica]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CEC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References