Detailed information of TA system TA235672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4520471..4521087
• Replicon: chromosome
• Accession: NZ_CP092075
• Organism: Salmonella enterica strain P281972

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8Z2U3
• Locus tag: MC377_RS22185
• Protein ID: WP_000238494.1
• Coordinates: 4520471..4520845 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8E9YHE9
• Locus tag: MC377_RS22190
• Protein ID: WP_001523745.1
• Coordinates: 4520845..4521087 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC377_RS22170 (4517973)	4517973..4518875	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
MC377_RS22175 (4518872)	4518872..4519507	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
MC377_RS22180 (4519504)	4519504..4520433	+	930	WP_000027734.1	formate dehydrogenase accessory protein FdhE	-
MC377_RS22185 (4520471)	4520471..4520845	-	375	WP_000238494.1	type II toxin-antitoxin system VapC family toxin	Toxin
MC377_RS22190 (4520845)	4520845..4521087	-	243	WP_001523745.1	CopG family transcriptional regulator	Antitoxin
MC377_RS22195 (4521292)	4521292..4522221	+	930	WP_058661473.1	alpha/beta hydrolase	-
MC377_RS22200 (4522307)	4522307..4522618	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
MC377_RS22205 (4522615)	4522615..4523061	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
MC377_RS22210 (4523076)	4523076..4524017	-	942	WP_238727967.1	fatty acid biosynthesis protein FabY	-
MC377_RS22215 (4524062)	4524062..4524499	-	438	WP_000560956.1	D-aminoacyl-tRNA deacylase	-
MC377_RS22220 (4524496)	4524496..4525368	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MC377_RS22225 (4525362)	4525362..4525961	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13908.18 Da
• Isoelectric Point: 7.3567

>T235672 WP_000238494.1 NZ_CP092075:c4520845-4520471 [Salmonella enterica]
MVKGSALFDTNILIDLFSGRIEAKHALEAYPPQNAISLITWMEVMVGAKKYHQENRTRIALSAFNIIGVTQEIAERSVIV
RQEYGMKLPDAIILATAQVHRCELVTRNTKDFADIPGVITPYHL

Nucleotide

• Length: 375 bp

>T235672 NZ_LR134321:c1662090-1661674 [Shewanella baltica]
TTGAATGACATTATTATCAACAAAATAGCAACCATCAAACGCTGTATAAAACGCATCCAACAAGTTTATGGTGATGGCAG
CATGTTCAAACAAGACTTCACCCTGCAAGATAGCGTTATCCTCAATTTACAACGCTGCTGCGAAGCCAGTATCGACATAG
CAAACCATATTAACCGCCAACAACAACTGGGTATCCCCCAAAGTAGCCGTGATAGCTTTACACTACTTTCACAAAATAAA
ATAATTAATCAGCAGCTTGCAGACAACTTAAAGAAGATGGTTGGCCTAAGAAACATCGCAGTACATGACTATCAAGAACT
CAACCTCGATATCGTTGTGCATGTTGTACAGCATCATCTTGAGGATTTTGAGCAATTTATTGAAGTGATAAAATTTTGTC
CAAGAATGAGTTATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.77 Da
• Isoelectric Point: 4.4463

>AT235672 WP_001523745.1 NZ_CP092075:c4521087-4520845 [Salmonella enterica]
MMAGMDMGRILLDLSDDVIKRLDDLKVQRNLPRAELLREAVEQYLERQDRAETTISKALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT235672 NZ_LR134321:c1662505-1662083 [Shewanella baltica]
ATGCAACAACTAAATGAAAGCATTATCATCAACTTACTACAAGATAACATCCCGCAGTTGAGATTGATCTACCTATTTGG
CTCCTATGCACAGGGCACGCAACACCGCAACAGTGATATCGATATTGCGGTACTCGCCGATGTCACTGTAGACAATATCG
CCCGCTGGCAACTTGCCCAAAAACTCGCCTCAGCGTTAGATACTGATGTTGATCTTGTTGATTTACGCACGGCATCCACA
GTGTTATGTCAGCAGGTAGTCACCCAAGGAAAGCGACTTTGGGGAACGCGACAAGATGATGAGATTTTTGCAGTGAAAAC
CATCTCAATGTACCAACATCTACAAGCTGAAAGACAAGCAATTATTGATGATGCAACGGCCAAAACAATAGCCAACGATC
ACAGGGGACGTCACTTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A5I6CND8