Detailed information of TA system TA235635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1298995..1299548
• Replicon: chromosome
• Accession: NZ_CP092070
• Organism: Pectobacterium sp. PL64

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HP572_RS05525
• Protein ID: WP_240585007.1
• Coordinates: 1299234..1299548 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A3S1FM98
• Locus tag: HP572_RS05520
• Protein ID: WP_029729596.1
• Coordinates: 1298995..1299231 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP572_RS05495 (HP572_05495)	1294733..1295794	-	1062	Protein_1075	zinc metallochaperone AztD	-
HP572_RS05500 (HP572_05500)	1295897..1296832	-	936	WP_240585004.1	zinc ABC transporter substrate-binding protein AztC	-
HP572_RS05505 (HP572_05505)	1296838..1297728	-	891	WP_240585005.1	zinc ABC transporter permease AztB	-
HP572_RS05510 (HP572_05510)	1297728..1298453	-	726	WP_240585006.1	zinc ABC transporter ATP-binding protein AztA	-
HP572_RS05515 (HP572_05515)	1298613..1298714	+	102	Protein_1079	DNA primase	-
HP572_RS05520 (HP572_05520)	1298995..1299231	+	237	WP_029729596.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HP572_RS05525 (HP572_05525)	1299234..1299548	+	315	WP_240585007.1	CcdB family protein	Toxin
HP572_RS05530 (HP572_05530)	1299582..1299683	-	102	Protein_1082	mobilization protein mobC	-
HP572_RS05535 (HP572_05535)	1300300..1300521	-	222	Protein_1083	IS3 family transposase	-
HP572_RS05540 (HP572_05540)	1300830..1301480	+	651	WP_240585008.1	hypothetical protein	-
HP572_RS05545 (HP572_05545)	1301618..1302424	+	807	WP_240585009.1	hypothetical protein	-
HP572_RS05550 (HP572_05550)	1302511..1302969	-	459	WP_240585819.1	hypothetical protein	-
HP572_RS05555 (HP572_05555)	1303063..1303385	+	323	Protein_1087	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11569.49 Da
• Isoelectric Point: 7.9859

>T235635 WP_240585007.1 NZ_CP092070:1299234-1299548 [Pectobacterium sp. PL64]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPDRFVPVIRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRSQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T235635 NZ_LR134315:c588749-588285 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8927.96 Da
• Isoelectric Point: 6.4612

>AT235635 WP_029729596.1 NZ_CP092070:1298995-1299231 [Pectobacterium sp. PL64]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTTALDAELRSHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT235635 NZ_LR134315:c589084-588749 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
ACCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A3S1FM98