Detailed information of TA system TA235634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1144085..1144638
• Replicon: chromosome
• Accession: NZ_CP092070
• Organism: Pectobacterium sp. PL64

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HP572_RS04825
• Protein ID: WP_240584915.1
• Coordinates: 1144324..1144638 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0H3I2T3
• Locus tag: HP572_RS04820
• Protein ID: WP_014699357.1
• Coordinates: 1144085..1144321 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP572_RS04815 (HP572_04815)	1142924..1143764	+	841	Protein_939	ArdC-like ssDNA-binding domain-containing protein	-
HP572_RS04820 (HP572_04820)	1144085..1144321	+	237	WP_014699357.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HP572_RS04825 (HP572_04825)	1144324..1144638	+	315	WP_240584915.1	CcdB family protein	Toxin
HP572_RS04830 (HP572_04830)	1145096..1145686	-	591	WP_240584916.1	DUF4326 domain-containing protein	-
HP572_RS04835 (HP572_04835)	1145683..1146123	-	441	WP_240584917.1	DUF488 domain-containing protein	-
HP572_RS04840 (HP572_04840)	1146140..1146814	-	675	WP_240584918.1	hypothetical protein	-
HP572_RS04845 (HP572_04845)	1147093..1147839	-	747	WP_240584919.1	MobC family replication-relaxation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1131157..1170066	38909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11563.53 Da
• Isoelectric Point: 7.9859

>T235634 WP_240584915.1 NZ_CP092070:1144324-1144638 [Pectobacterium sp. PL64]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPDRLVPLIRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRTQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T235634 NZ_LR134315:263653-263895 [Escherichia coli]
CTGAGTGTTGTATCTAATCGTGGGGGCAGGTCACCTGTTCCTCAAGGGATCAAGTGGTCTGATATAGAGTCACTGGTTAA
AGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATC
GGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCA
TGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9004.06 Da
• Isoelectric Point: 6.4610

>AT235634 WP_014699357.1 NZ_CP092070:1144085-1144321 [Pectobacterium sp. PL64]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTTALDAELRYHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT235634 NZ_LR134315:263892-264251 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3I2T3