Detailed information of TA system TA235626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2150205..2151925
• Replicon: chromosome
• Accession: NZ_CP092065
• Organism: Edwardsiella ictaluri strain S15-163

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: C5BBX2
• Locus tag: MAY82_RS10400
• Protein ID: WP_015872652.1
• Coordinates: 2150205..2150861 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: MAY82_RS10405
• Protein ID: WP_278174736.1
• Coordinates: 2150858..2151925 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAY82_RS10380 (MAY82_10330)	2145328..2146079	+	752	WP_226092439.1	IS5 family transposase	-
MAY82_RS10385 (MAY82_10335)	2146160..2146399	+	240	WP_015872655.1	hypothetical protein	-
MAY82_RS10390 (MAY82_10340)	2146438..2148876	+	2439	WP_015872654.1	type I restriction-modification system subunit M	-
MAY82_RS10395 (MAY82_10345)	2148876..2150192	+	1317	WP_226092363.1	restriction endonuclease subunit S	-
MAY82_RS10400 (MAY82_10350)	2150205..2150861	+	657	WP_015872652.1	DUF4433 domain-containing protein	Toxin
MAY82_RS10405 (MAY82_10355)	2150858..2151925	+	1068	WP_278174736.1	macro domain-containing protein	Antitoxin
MAY82_RS10410 (MAY82_10360)	2152089..2155184	+	3096	WP_015872650.1	HsdR family type I site-specific deoxyribonuclease	-
MAY82_RS10415 (MAY82_10365)	2155184..2155900	+	717	WP_015872649.1	SprT family zinc-dependent metalloprotease	-
MAY82_RS10420 (MAY82_10370)	2156131..2156685	-	555	WP_015872648.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2144551..2159569	15018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25178.69 Da
• Isoelectric Point: 6.9647

>T235626 WP_015872652.1 NZ_CP092065:2150205-2150861 [Edwardsiella ictaluri]
MAYDYSANLNPEKALIWRIVHRDNIPWILDHGLHCGNSPVQAANWINIGNPELIGKRAGHPVPVGMGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSNLRNVAAQNIPFVFTDSHAYYNWVNYYTRLDDLNQIDWSILQARDFRRDPDD
PAKFERYQAEALIWKHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T235626 NZ_LR134312:2624205-2624345 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40547.69 Da
• Isoelectric Point: 5.9391

>AT235626 WP_278174736.1 NZ_CP092065:2150858-2151925 [Edwardsiella ictaluri]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYADACKQKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMAWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTEKYQNVAKSS
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDRGKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEG
LHQWPAGEPWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT235626 NZ_LR134312:c2624203-2624060 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAA

Structures

Toxin

• AlphaFold DB: C5BBX2