Detailed information of TA system TA235625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 795502..796165
• Replicon: chromosome
• Accession: NZ_CP092065
• Organism: Edwardsiella ictaluri strain S15-163

Toxin (Protein)

• Gene name: tad
• Uniprot ID: C5B7U4
• Locus tag: MAY82_RS03950
• Protein ID: WP_015870280.1
• Coordinates: 795502..795846 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: MAY82_RS03955
• Protein ID: WP_015870279.1
• Coordinates: 795848..796165 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAY82_RS03930 (MAY82_03905)	791626..792270	-	645	WP_226092411.1	phosphatidylglycerophosphatase C	-
MAY82_RS03935 (MAY82_03910)	792426..793273	+	848	Protein_758	MurR/RpiR family transcriptional regulator	-
MAY82_RS03940 (MAY82_03915)	794400..795083	-	684	WP_107775187.1	phage portal protein	-
MAY82_RS03945 (MAY82_03920)	795112..795330	+	219	WP_232347353.1	head completion/stabilization protein	-
MAY82_RS03950 (MAY82_03925)	795502..795846	+	345	WP_015870280.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MAY82_RS03955 (MAY82_03930)	795848..796165	+	318	WP_015870279.1	helix-turn-helix transcriptional regulator	Antitoxin
MAY82_RS03960 (MAY82_03935)	796198..796575	-	378	WP_015870278.1	type II toxin-antitoxin system death-on-curing family toxin	-
MAY82_RS03965	796572..796700	-	129	WP_015870277.1	hypothetical protein	-
MAY82_RS03970 (MAY82_03940)	796805..796984	-	180	WP_071525714.1	hypothetical protein	-
MAY82_RS03975 (MAY82_03945)	797213..797964	+	752	WP_226092409.1	IS5 family transposase	-
MAY82_RS03980 (MAY82_03950)	797930..798214	-	285	WP_050977424.1	hypothetical protein	-
MAY82_RS03985 (MAY82_03955)	798813..799073	+	261	WP_015870274.1	YfhL family 4Fe-4S dicluster ferredoxin	-
MAY82_RS03990 (MAY82_03960)	799095..799772	-	678	WP_015870273.1	envelope stress response activation lipoprotein NlpE	-
MAY82_RS03995 (MAY82_03965)	799869..800285	-	417	WP_015870272.1	alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB	-
MAY82_RS04000 (MAY82_03970)	800287..800836	-	550	Protein_771	YaeQ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-86)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12544.34 Da
• Isoelectric Point: 9.8474

>T235625 WP_015870280.1 NZ_CP092065:795502-795846 [Edwardsiella ictaluri]
MKSLYWVGSTKKDLQSLPEEVQDIFGYALHLAQVGGKHSQTKPLKGFSGAGVLEVVEDFLGDTYRAVYTVKFGDAVYVSH
VFQKKSSSGIATPKLNMDKIRERLKAAENHARGA

Nucleotide

• Length: 345 bp

>T235625 NZ_LR134312:2623943-2624086 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11480.41 Da
• Isoelectric Point: 10.2058

>AT235625 WP_015870279.1 NZ_CP092065:795848-796165 [Edwardsiella ictaluri]
MSHDIEVSSGNVYVDIGRDDAEEMLLKAQLATTIGNIIKSRSLTQEQAAKLLGMTQPKLSNMLRGQFRGISEAKMLECLT
RLGRDVQIVVGKPRRTPGSVKVVLA

Nucleotide

• Length: 318 bp

>AT235625 NZ_LR134312:c2624203-2624019 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: C5B7U4