Detailed information of TA system TA235620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2159051..2160771
• Replicon: chromosome
• Accession: NZ_CP092062
• Organism: Edwardsiella ictaluri strain S00-1912

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: C5BBX2
• Locus tag: MAY76_RS10390
• Protein ID: WP_015872652.1
• Coordinates: 2159051..2159707 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: C5BBX1
• Locus tag: MAY76_RS10395
• Protein ID: WP_015872651.1
• Coordinates: 2159704..2160771 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAY76_RS10370 (MAY76_10300)	2154175..2154925	+	751	WP_169315013.1	IS5 family transposase	-
MAY76_RS10375 (MAY76_10305)	2155006..2155245	+	240	WP_015872655.1	hypothetical protein	-
MAY76_RS10380 (MAY76_10310)	2155284..2157722	+	2439	WP_015872654.1	type I restriction-modification system subunit M	-
MAY76_RS10385 (MAY76_10315)	2157722..2159038	+	1317	WP_226092363.1	restriction endonuclease subunit S	-
MAY76_RS10390 (MAY76_10320)	2159051..2159707	+	657	WP_015872652.1	DUF4433 domain-containing protein	Toxin
MAY76_RS10395 (MAY76_10325)	2159704..2160771	+	1068	WP_015872651.1	macro domain-containing protein	Antitoxin
MAY76_RS10400 (MAY76_10330)	2160935..2164030	+	3096	WP_015872650.1	HsdR family type I site-specific deoxyribonuclease	-
MAY76_RS10405 (MAY76_10335)	2164030..2164746	+	717	WP_015872649.1	SprT family zinc-dependent metalloprotease	-
MAY76_RS10410 (MAY76_10340)	2164977..2165531	-	555	WP_015872648.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2153398..2168415	15017

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25178.69 Da
• Isoelectric Point: 6.9647

>T235620 WP_015872652.1 NZ_CP092062:2159051-2159707 [Edwardsiella ictaluri]
MAYDYSANLNPEKALIWRIVHRDNIPWILDHGLHCGNSPVQAANWINIGNPELIGKRAGHPVPVGMGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSNLRNVAAQNIPFVFTDSHAYYNWVNYYTRLDDLNQIDWSILQARDFRRDPDD
PAKFERYQAEALIWKHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T235620 NZ_LR134311:c4651401-4651090 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40619.75 Da
• Isoelectric Point: 5.6965

>AT235620 WP_015872651.1 NZ_CP092062:2159704-2160771 [Edwardsiella ictaluri]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYADACKQKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMAWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTEKYQNVAKSS
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEG
LHQWPAGEPWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT235620 NZ_LR134311:c4651089-4650799 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: C5BBX2

Antitoxin

• AlphaFold DB: C5BBX1