Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4444753..4445250 Replicon chromosome
Accession NC_011993
Organism Escherichia coli LF82

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LF82_RS21905 Protein ID WP_001173343.1
Coordinates 4445077..4445250 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag LF82_RS21900 Protein ID WP_001312331.1
Coordinates 4444753..4445049 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LF82_RS21880 4441482..4442201 - 720 WP_000611291.1 two-component system response regulator DcuR -
LF82_RS21885 4442198..4443829 - 1632 WP_001216466.1 sensor histidine kinase -
LF82_RS21890 4444010..4444240 + 231 WP_000373294.1 (4Fe-4S)-binding protein -
LF82_RS21895 4444252..4444524 + 273 WP_000405647.1 N-acetyltransferase -
LF82_RS21900 4444753..4445049 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LF82_RS21905 4445077..4445250 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LF82_RS21910 4445369..4446886 - 1518 WP_000003805.1 lysine--tRNA ligase -
LF82_RS21915 4447123..4448580 - 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T23562 WP_001173343.1 NC_011993:4445077-4445250 [Escherichia coli LF82]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T23562 NC_011993:4445077-4445250 [Escherichia coli LF82]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT23562 WP_001312331.1 NC_011993:4444753-4445049 [Escherichia coli LF82]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT23562 NC_011993:4444753-4445049 [Escherichia coli LF82]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References