Detailed information of TA system TA235619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 804262..804925
• Replicon: chromosome
• Accession: NZ_CP092062
• Organism: Edwardsiella ictaluri strain S00-1912

Toxin (Protein)

• Gene name: tad
• Uniprot ID: C5B7U4
• Locus tag: MAY76_RS03960
• Protein ID: WP_015870280.1
• Coordinates: 804262..804606 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: MAY76_RS03965
• Protein ID: WP_015870279.1
• Coordinates: 804608..804925 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAY76_RS03940 (MAY76_03905)	800385..801007	-	623	Protein_759	phosphatidylglycerophosphatase C	-
MAY76_RS03945 (MAY76_03910)	801184..802031	+	848	Protein_760	MurR/RpiR family transcriptional regulator	-
MAY76_RS03950 (MAY76_03915)	803160..803843	-	684	WP_107775187.1	phage portal protein	-
MAY76_RS03955 (MAY76_03920)	803872..804090	+	219	WP_232347353.1	head completion/stabilization protein	-
MAY76_RS03960 (MAY76_03925)	804262..804606	+	345	WP_015870280.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MAY76_RS03965 (MAY76_03930)	804608..804925	+	318	WP_015870279.1	helix-turn-helix transcriptional regulator	Antitoxin
MAY76_RS03970 (MAY76_03935)	804958..805335	-	378	WP_015870278.1	type II toxin-antitoxin system death-on-curing family toxin	-
MAY76_RS03975	805332..805460	-	129	WP_015870277.1	hypothetical protein	-
MAY76_RS03980 (MAY76_03940)	805565..805744	-	180	WP_071525714.1	hypothetical protein	-
MAY76_RS03985 (MAY76_03945)	805972..806723	+	752	WP_226092409.1	IS5 family transposase	-
MAY76_RS03990 (MAY76_03950)	806689..806973	-	285	WP_050977424.1	hypothetical protein	-
MAY76_RS03995 (MAY76_03955)	807573..807833	+	261	WP_015870274.1	YfhL family 4Fe-4S dicluster ferredoxin	-
MAY76_RS04000 (MAY76_03960)	807855..808532	-	678	WP_015870273.1	envelope stress response activation lipoprotein NlpE	-
MAY76_RS04005 (MAY76_03965)	808628..809042	-	415	Protein_772	alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB	-
MAY76_RS04010 (MAY76_03970)	809044..809592	-	549	WP_015870271.1	YaeQ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	795075..805335	10260

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-86)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12544.34 Da
• Isoelectric Point: 9.8474

>T235619 WP_015870280.1 NZ_CP092062:804262-804606 [Edwardsiella ictaluri]
MKSLYWVGSTKKDLQSLPEEVQDIFGYALHLAQVGGKHSQTKPLKGFSGAGVLEVVEDFLGDTYRAVYTVKFGDAVYVSH
VFQKKSSSGIATPKLNMDKIRERLKAAENHARGA

Nucleotide

• Length: 345 bp

>T235619 NZ_LR134311:c4374326-4374153 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11480.41 Da
• Isoelectric Point: 10.2058

>AT235619 WP_015870279.1 NZ_CP092062:804608-804925 [Edwardsiella ictaluri]
MSHDIEVSSGNVYVDIGRDDAEEMLLKAQLATTIGNIIKSRSLTQEQAAKLLGMTQPKLSNMLRGQFRGISEAKMLECLT
RLGRDVQIVVGKPRRTPGSVKVVLA

Nucleotide

• Length: 318 bp

>AT235619 NZ_LR134311:c4374650-4374354 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: C5B7U4