Detailed information of TA system TA235614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2030137..2030666
• Replicon: chromosome
• Accession: NZ_CP092055
• Organism: Staphylococcus aureus strain USA600

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AH5611_RS10000
• Protein ID: WP_000621175.1
• Coordinates: 2030137..2030499 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AH5611_RS10005
• Protein ID: WP_000948331.1
• Coordinates: 2030496..2030666 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AH5611_RS09980 (2027115)	2027115..2027885	-	771	WP_238762296.1	RNA polymerase sigma factor SigB	-
AH5611_RS09985 (2027860)	2027860..2028339	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
AH5611_RS09990 (2028341)	2028341..2028667	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AH5611_RS09995 (2028786)	2028786..2029787	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AH5611_RS10000 (2030137)	2030137..2030499	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AH5611_RS10005 (2030496)	2030496..2030666	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AH5611_RS10010 (2030751)	2030751..2031899	-	1149	WP_001281140.1	alanine racemase	-
AH5611_RS10015 (2031965)	2031965..2032324	-	360	WP_000581199.1	holo-ACP synthase	-
AH5611_RS10020 (2032328)	2032328..2032819	-	492	WP_001205907.1	PH domain-containing protein	-
AH5611_RS10025 (2032806)	2032806..2034389	-	1584	WP_001294637.1	PH domain-containing protein	-
AH5611_RS10030 (2034382)	2034382..2034861	-	480	WP_001287077.1	hypothetical protein	-
AH5611_RS10035 (2035069)	2035069..2035629	-	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T235614 WP_000621175.1 NZ_CP092055:c2030499-2030137 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T235614 NZ_LR134311:3527997-3528215 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT235614 WP_000948331.1 NZ_CP092055:c2030666-2030496 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT235614 NZ_LR134311:3527597-3527971 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1