Detailed information of TA system TA235603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2119155..2119684
• Replicon: chromosome
• Accession: NZ_CP092052
• Organism: Staphylococcus aureus strain USA300

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AH5667_RS10605
• Protein ID: WP_000621175.1
• Coordinates: 2119155..2119517 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AH5667_RS10610
• Protein ID: WP_000948331.1
• Coordinates: 2119514..2119684 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AH5667_RS10585 (2116133)	2116133..2116903	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
AH5667_RS10590 (2116878)	2116878..2117357	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
AH5667_RS10595 (2117359)	2117359..2117685	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AH5667_RS10600 (2117804)	2117804..2118805	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AH5667_RS10605 (2119155)	2119155..2119517	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AH5667_RS10610 (2119514)	2119514..2119684	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AH5667_RS10615 (2119769)	2119769..2120917	-	1149	WP_001281145.1	alanine racemase	-
AH5667_RS10620 (2120983)	2120983..2121342	-	360	WP_000581200.1	holo-ACP synthase	-
AH5667_RS10625 (2121346)	2121346..2121837	-	492	WP_001205910.1	PH domain-containing protein	-
AH5667_RS10630 (2121824)	2121824..2123407	-	1584	WP_001294626.1	PH domain-containing protein	-
AH5667_RS10635 (2123400)	2123400..2123879	-	480	WP_001287088.1	hypothetical protein	-
AH5667_RS10640 (2124087)	2124087..2124647	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T235603 WP_000621175.1 NZ_CP092052:c2119517-2119155 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T235603 NZ_LR134311:c788980-788603 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT235603 WP_000948331.1 NZ_CP092052:c2119684-2119514 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT235603 NZ_LR134311:c789401-789027 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCTCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGATCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1