Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 31052..31695 Replicon plasmid pAVS0889-b
Accession NZ_CP092044
Organism Enterobacter cloacae isolate AVS0889

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag MBA36_RS24390 Protein ID WP_000754567.1
Coordinates 31279..31695 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag MBA36_RS24385 Protein ID WP_023293777.1
Coordinates 31052..31282 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA36_RS24360 (MBA36_24360) 26252..26683 + 432 WP_006796638.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
MBA36_RS24365 (MBA36_24365) 26683..27954 + 1272 WP_238518569.1 Y-family DNA polymerase -
MBA36_RS24370 (MBA36_24370) 28036..29010 - 975 WP_000064119.1 ParB family protein -
MBA36_RS24375 (MBA36_24375) 29010..30215 - 1206 WP_006797591.1 AAA family ATPase -
MBA36_RS24380 (MBA36_24380) 30345..30416 + 72 Protein_28 hypothetical protein -
MBA36_RS24385 (MBA36_24385) 31052..31282 + 231 WP_023293777.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MBA36_RS24390 (MBA36_24390) 31279..31695 + 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
MBA36_RS24395 (MBA36_24395) 32046..33014 - 969 WP_084832609.1 IS5-like element IS903B family transposase -
MBA36_RS24400 (MBA36_24400) 33140..33841 - 702 WP_008502228.1 DsbA family oxidoreductase -
MBA36_RS24405 (MBA36_24405) 33907..35013 - 1107 WP_006687059.1 alkene reductase -
MBA36_RS24410 (MBA36_24410) 35226..35555 + 330 WP_008502229.1 thioredoxin family protein -
MBA36_RS24415 (MBA36_24415) 35585..35923 - 339 WP_000888080.1 carboxymuconolactone decarboxylase family protein -
MBA36_RS24420 (MBA36_24420) 35928..36509 - 582 WP_032676110.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-10 - 1..90827 90827


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T235591 WP_000754567.1 NZ_CP092044:31279-31695 [Enterobacter cloacae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T235591 NZ_LR134309:c149000-148719 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT235591 WP_023293777.1 NZ_CP092044:31052-31282 [Enterobacter cloacae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT235591 NZ_LR134309:c149224-148997 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References