Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5011..5747 Replicon plasmid pAVS0889-b
Accession NZ_CP092044
Organism Enterobacter cloacae isolate AVS0889

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MBA36_RS24270 Protein ID WP_003026803.1
Coordinates 5265..5747 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MBA36_RS24265 Protein ID WP_003026799.1
Coordinates 5011..5277 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA36_RS24245 (MBA36_24245) 1709..2449 - 741 WP_001515717.1 tyrosine-type recombinase/integrase -
MBA36_RS24250 (MBA36_24250) 2813..3781 - 969 WP_023307223.1 IS5-like element IS903B family transposase -
MBA36_RS24255 (MBA36_24255) 4119..4322 + 204 WP_004213596.1 HHA domain-containing protein -
MBA36_RS24260 (MBA36_24260) 4336..4566 + 231 WP_023307222.1 hypothetical protein -
MBA36_RS24265 (MBA36_24265) 5011..5277 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MBA36_RS24270 (MBA36_24270) 5265..5747 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MBA36_RS24275 (MBA36_24275) 5906..7438 - 1533 WP_015065592.1 IS3-like element ISKpn38 family transposase -
MBA36_RS24280 (MBA36_24280) 7561..8904 + 1344 WP_080339306.1 ISNCY family transposase -
MBA36_RS24285 (MBA36_24285) 8975..9793 - 819 WP_023307220.1 abortive infection family protein -
MBA36_RS24290 (MBA36_24290) 9802..10374 - 573 WP_032676095.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-10 - 1..90827 90827
- inside IScluster/Tn mcr-10 - 2813..17865 15052


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T235590 WP_003026803.1 NZ_CP092044:5265-5747 [Enterobacter cloacae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T235590 NZ_LR134308:6555500-6555778 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGGCTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCACAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT235590 WP_003026799.1 NZ_CP092044:5011-5277 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT235590 NZ_LR134308:6555790-6556095 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References