Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1125248..1125868 Replicon chromosome
Accession NZ_CP092042
Organism Enterobacter cloacae isolate AVS0889

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag MBA36_RS05250 Protein ID WP_013095889.1
Coordinates 1125248..1125466 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag MBA36_RS05255 Protein ID WP_008499288.1
Coordinates 1125494..1125868 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA36_RS05220 (MBA36_05220) 1121260..1121520 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
MBA36_RS05225 (MBA36_05225) 1121523..1121663 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MBA36_RS05230 (MBA36_05230) 1121660..1122370 - 711 WP_013095885.1 GNAT family protein -
MBA36_RS05235 (MBA36_05235) 1122472..1123932 + 1461 WP_013095886.1 PLP-dependent aminotransferase family protein -
MBA36_RS05240 (MBA36_05240) 1123904..1124371 - 468 WP_013095887.1 YlaC family protein -
MBA36_RS05245 (MBA36_05245) 1124487..1125038 - 552 WP_013095888.1 maltose O-acetyltransferase -
MBA36_RS05250 (MBA36_05250) 1125248..1125466 - 219 WP_013095889.1 HHA domain-containing protein Toxin
MBA36_RS05255 (MBA36_05255) 1125494..1125868 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
MBA36_RS05260 (MBA36_05260) 1126381..1129527 - 3147 WP_013095890.1 multidrug efflux RND transporter permease subunit AcrB -
MBA36_RS05265 (MBA36_05265) 1129550..1130743 - 1194 WP_023620093.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T235577 WP_013095889.1 NZ_CP092042:c1125466-1125248 [Enterobacter cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T235577 NZ_LR134306:3706974-3707177 [Yersinia pseudotuberculosis]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATCGATACGCTAGAACGTGTAATTGAAAAAAATAA
GTACGAACTTTCTGATGATGAACTGGAATTGTTTTACTCAGCTGCAGACCATCGCCTGGCTGAACTAACGATGAATAAGC
TTTATGATAAAATTCCACCTACTGTATGGCAACATGTGAAATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT235577 WP_008499288.1 NZ_CP092042:c1125868-1125494 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT235577 NZ_LR134306:3706557-3706925 [Yersinia pseudotuberculosis]
ATGGATGAGTACTCGCCTAAGCGGCATGACATTGCCCAACTGAAGTTTCTATGTGAAAGCTTGTATGATGAAGGTATTGC
AACTCTTGGCGACAGCCATCATGGCTGGATAAATGACCCAACGTCGGCCATAAACCTTCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGGAAGCCAGCTTTCCGAGTTAGTGGAAGAATATTTA
GACGACACATATACTCTGTTTAGTAGTTATGGAATCAATGATCCTGAATTGCGTCGCTGGCAGAAAACCAAAGAGCATTT
ATTCAGACTTTTCTCAGGGGAATACGTATGTACCCTGATGAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References