Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 593676..594470 Replicon chromosome
Accession NZ_CP092042
Organism Enterobacter cloacae isolate AVS0889

Toxin (Protein)


Gene name KacT Uniprot ID A0A7V7JFS7
Locus tag MBA36_RS02840 Protein ID WP_013095427.1
Coordinates 593676..594197 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A5E1AP76
Locus tag MBA36_RS02845 Protein ID WP_013095428.1
Coordinates 594201..594470 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA36_RS02815 (MBA36_02815) 589003..589164 + 162 WP_013095422.1 DUF1127 domain-containing protein -
MBA36_RS02820 (MBA36_02820) 589251..590639 - 1389 WP_013095423.1 PLP-dependent aminotransferase family protein -
MBA36_RS02825 (MBA36_02825) 590742..591221 + 480 WP_013095424.1 carboxymuconolactone decarboxylase family protein -
MBA36_RS02830 (MBA36_02830) 591208..592110 - 903 WP_013095425.1 LysR family transcriptional regulator -
MBA36_RS02835 (MBA36_02835) 592211..593626 + 1416 WP_013095426.1 aldehyde dehydrogenase family protein -
MBA36_RS02840 (MBA36_02840) 593676..594197 - 522 WP_013095427.1 GNAT family N-acetyltransferase Toxin
MBA36_RS02845 (MBA36_02845) 594201..594470 - 270 WP_013095428.1 DUF1778 domain-containing protein Antitoxin
MBA36_RS02850 (MBA36_02850) 594685..595008 + 324 WP_023620659.1 antibiotic biosynthesis monooxygenase -
MBA36_RS02855 (MBA36_02855) 595001..595399 + 399 WP_013095430.1 amino acid-binding protein -
MBA36_RS02860 (MBA36_02860) 595371..596066 + 696 WP_013095431.1 winged helix-turn-helix domain-containing protein -
MBA36_RS02865 (MBA36_02865) 596270..596740 + 471 WP_013095432.1 MarR family transcriptional regulator -
MBA36_RS02870 (MBA36_02870) 596737..597804 + 1068 WP_095440265.1 HlyD family secretion protein -
MBA36_RS02875 (MBA36_02875) 597794..598870 + 1077 WP_013095434.1 DUF2955 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-150)

Antitoxin

Vibrio phage ICP1, Orf50

(3-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19410.33 Da        Isoelectric Point: 9.2434

>T235576 WP_013095427.1 NZ_CP092042:c594197-593676 [Enterobacter cloacae]
VNNMKIGIFSDDVEYDLSHFDCGEESLNTFLTAHLKRQHRGKFLRGYVLVASGEKPRVLGYYTLSGSCFEKAYLPSKTQQ
KRVPYKNVPSVTLGRLAIDKRFQGQGLGELLVTHALKTVYLASFAVGIHGIFVEALNDSAKNFYLKLGFIALQAENENTL
FLPTKTIERLFAD

Download         Length: 522 bp

>T235576 NZ_LR134306:c3614109-3613789 [Yersinia pseudotuberculosis]
ATGAATATCTCAACTGTACCGGCCACGGTGCCGGTTTCGTCACGCCTGTCACCCGTTCAAGTCTGGCAGCAGCTGTTAAC
CTATTTGCTGGAACAGCATTATGGCCTCACGTTAAACGACACACCCTTTCATGACGATACAACTATCCAGGAACATATTG
AAGCGGGAATAACGCTTGCTGATGCCGTGAATTTTCTGGTGGAACGCTATGAGCTGGTACGTATTGATCGCAAAGGTTTT
ACCTGGCAGGAGCAGAGTCCCTTTCTGACGGCAACCGATATGCTCAGAGCCAGACGCGCTACCGGTCTAATCAACACCTG
A

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10177.54 Da        Isoelectric Point: 5.8652

>AT235576 WP_013095428.1 NZ_CP092042:c594470-594201 [Enterobacter cloacae]
MSALKKQRIDLRLTEDDKSLIEEAAAMTNQSISQFMVSTASERAAEVIEQHRRLILNEASWNQVMDAISNPPTPNERLKR
AARRLQDLE

Download         Length: 270 bp

>AT235576 NZ_LR134306:c3614469-3614146 [Yersinia pseudotuberculosis]
ATGCCATTAACGTCACCCCATGAATGGGGGTTACAAATCGATGTCACCCCGCGCTTAGGTGCAAGGCTGGTTCAGGAGGG
CAATCGGCTGCACTATCTGGCAGACAGAGCTGGATTAACGGGAAACTTTAGCCCAGAGCAATTACAACAACTGGAACGGA
CATTCTCGCTATTTATCGGGCAGATGGAAGGAATGCTGTATTCCGGTGAGCTAAATCCACATCAACAACATCAGGTGGCT
ATTCAGCTTGCGGGTTTAACCTGCATAGCGGATACACGTGCCAGCTGCGGCTACGTTTACATTTCTATTTATCCAATTCA
GTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7JFS7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AP76

References