Detailed information of TA system TA235542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4353492..4354276
• Replicon: chromosome
• Accession: NZ_CP092039
• Organism: Pectobacterium brasiliense strain 130

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: MBA20_RS19100
• Protein ID: WP_119871144.1
• Coordinates: 4353492..4353986 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A3S0Y1Y3
• Locus tag: MBA20_RS19105
• Protein ID: WP_038921611.1
• Coordinates: 4353983..4354276 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MBA20_RS19075 (MBA20_19075)	4348889..4349632	+	744	WP_119871148.1	MobC family replication-relaxation protein	-
MBA20_RS19080 (MBA20_19080)	4349691..4349909	+	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	-
MBA20_RS19085 (MBA20_19085)	4349912..4350217	+	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	-
MBA20_RS19090 (MBA20_19090)	4350417..4351259	-	843	WP_119871146.1	DNA adenine methylase	-
MBA20_RS19095 (MBA20_19095)	4351345..4353066	-	1722	WP_119871145.1	AAA family ATPase	-
MBA20_RS19100 (MBA20_19100)	4353492..4353986	-	495	WP_119871144.1	GNAT family N-acetyltransferase	Toxin
MBA20_RS19105 (MBA20_19105)	4353983..4354276	-	294	WP_038921611.1	DUF1778 domain-containing protein	Antitoxin
MBA20_RS19110 (MBA20_19110)	4354616..4354858	-	243	WP_072010299.1	helix-turn-helix transcriptional regulator	-
MBA20_RS19115 (MBA20_19115)	4354962..4355216	+	255	WP_119871224.1	helix-turn-helix transcriptional regulator	-
MBA20_RS19120 (MBA20_19120)	4355301..4355549	+	249	WP_119871143.1	helix-turn-helix transcriptional regulator	-
MBA20_RS19125 (MBA20_19125)	4356202..4357956	-	1755	WP_119871142.1	AIPR family protein	-
MBA20_RS19130 (MBA20_19130)	4358184..4358969	-	786	WP_185893700.1	phosphonate metabolism protein PhnP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4345716..4354532	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17689.53 Da
• Isoelectric Point: 7.2150

>T235542 WP_119871144.1 NZ_CP092039:c4353986-4353492 [Pectobacterium brasiliense]
MISAPEPLRAEHILSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T235542 NZ_LR134302:c1931031-1930744 [Achromobacter spanius]
GTGCCGGTTCTTGAATGGCGCGAAACGGCTCGCGAGGATTTGCTGGCGATTGTCGATTACATCTCGGACGATAGCCCGGC
CGCGGCGCTGCGTTTGGTAGATGAAATTCAGAAGAAAGTGGTCAAGCTGCGTGAGCGCCCACGGCTGTATCGCGCGGGAC
GGGTAGCGGGCACCAGGGAAATGGTGATTCGGCCGAACTATTTGGTTGTGTATGCGGAATCGCCTCGCGCCGTGACAATT
TTGCGCGTCCTGCACGCCGCGCAGCAATGGCCGCTCGATAGCGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10856.44 Da
• Isoelectric Point: 10.1968

>AT235542 WP_038921611.1 NZ_CP092039:c4354276-4353983 [Pectobacterium brasiliense]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT235542 NZ_LR134302:c1931317-1931018 [Achromobacter spanius]
ATGGCACCCACTTCAATGTTGCACGTCCGTGTCGACGACAAGCTCAAGGCCGAAGCGGCGGAAAAGCTCGCCAACGTTGG
CCTGACCATCTCGGATGCGGTGCGAATTTTGCTGACAAGAGTGGCAAAAGAAGGCGGTTTGCCCGCCGGCTTGGCCGTCG
ACGCCTTGAGCTACGACTCTTGGTTTCGCGCGAAGGTGCAAGAGGCCTTGGCCGATACGCGGCCACCGGTCGCCCATGAA
GCGGTCATGGACGAGGCTCGTGCCCTGATCAGCAAGAAGAAGCGCCGTGCCGGTTCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S0Y1Y3