Detailed information of TA system TA235541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4349691..4350217
• Replicon: chromosome
• Accession: NZ_CP092039
• Organism: Pectobacterium brasiliense strain 130

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: MBA20_RS19085
• Protein ID: WP_119871147.1
• Coordinates: 4349912..4350217 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: MBA20_RS19080
• Protein ID: WP_015855076.1
• Coordinates: 4349691..4349909 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MBA20_RS19055 (MBA20_19055)	4345893..4347155	+	1263	WP_119871150.1	integrase arm-type DNA-binding domain-containing protein	-
MBA20_RS19060 (MBA20_19060)	4347271..4347465	-	195	WP_044209559.1	YlcI/YnfO family protein	-
MBA20_RS19065 (MBA20_19065)	4347651..4347992	+	342	WP_119871225.1	hypothetical protein	-
MBA20_RS19070 (MBA20_19070)	4348446..4348796	-	351	WP_158586005.1	hypothetical protein	-
MBA20_RS19075 (MBA20_19075)	4348889..4349632	+	744	WP_119871148.1	MobC family replication-relaxation protein	-
MBA20_RS19080 (MBA20_19080)	4349691..4349909	+	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
MBA20_RS19085 (MBA20_19085)	4349912..4350217	+	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	Toxin
MBA20_RS19090 (MBA20_19090)	4350417..4351259	-	843	WP_119871146.1	DNA adenine methylase	-
MBA20_RS19095 (MBA20_19095)	4351345..4353066	-	1722	WP_119871145.1	AAA family ATPase	-
MBA20_RS19100 (MBA20_19100)	4353492..4353986	-	495	WP_119871144.1	GNAT family N-acetyltransferase	-
MBA20_RS19105 (MBA20_19105)	4353983..4354276	-	294	WP_038921611.1	DUF1778 domain-containing protein	-
MBA20_RS19110 (MBA20_19110)	4354616..4354858	-	243	WP_072010299.1	helix-turn-helix transcriptional regulator	-
MBA20_RS19115 (MBA20_19115)	4354962..4355216	+	255	WP_119871224.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4345716..4354532	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11759.63 Da
• Isoelectric Point: 4.6533

>T235541 WP_119871147.1 NZ_CP092039:4349912-4350217 [Pectobacterium brasiliense]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIEAYHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVT
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T235541 NZ_LR134302:c1368408-1368133 [Achromobacter spanius]
ATGGAACTGAAATGGACCAGCAAGGCGCTTTCCGATCTGGCGCCCTTGTATGAATTCCTGGCCCCGGCGAATGCGCCCGC
AGCCGCGCGCGCGGTCCAGGCGCTGGTAAAAGCGCCCGGCATATTGCTGACCCATCCCCGTATTGGCGAACAGCTATTCC
AGTTCGAACCGCGAGAGGTTCGAAAAATACTCGTCGGGCAGTTTGAGGTGCGCTACGAAATTCAGGGTTCGATCATCTAC
GTGTTGCGGCTGTGGCACAGCCGAGAGGAACGCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT235541 WP_015855076.1 NZ_CP092039:4349691-4349909 [Pectobacterium brasiliense]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT235541 NZ_LR134302:c1368674-1368408 [Achromobacter spanius]
ATGCCCGTAGCCACCAAGGTGCTGACCGCACACGTGCCGCTCCCGCTGGCCGAGAAGGTAGATCAGATGGCTGCTCGTCT
TGAACGCTCGCGCGGCTGGATCATGAAGCAGGCGCTTTCCGCTTGGATCGCACAAGAGGAAGAACGCGATCGACTTACCC
AGGAAGCGCTGGCTGATGTCGAGGCCGGTCGCGTGGTTGACCACCAGGCCGTGCAAGCGTGGGCAGAAAGTCTGGGTACC
GACAAGCCGTTGCCGGTACCCCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46